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EC number: 407-230-4 | CAS number: 126637-70-5 BLUE GS 1259 R5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-05-16 to 1990-08-17
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study performed under the appropriate guideline during that time. The EC-50 results reported are referring to a testing time of 24 hours, whereas the new guideline requests to report data after 48 hours of exposure of daphnia to the test item. ECHA's endpoint specific guidance, chapter R.7B 8p49) says "...Invertebrates: ...standard testing data might be substituted by... a 24 hour EC50 value from short-term toxicity testing on Daphnia sp. according to OECD 202, but this should only be used in conjunction with other data (e.g. on time-dependence of toxicity) as part of a Weight of Evidence approach." Considering the overall quality of the study and consistency of the data obtained, and since the results from the test item at 24 hours can be compared with 24-hours toxicity data from the positive control substance, the study is considered as a Klimisch-2 study with acceptable restrictions, which eventually can qualify the study as a key study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- adopted April 4, 1984
- Deviations:
- yes
- Remarks:
- During that time OECD 202 requested to report EC50 after 24 hours, instead of EC50 48 hours as requested in the new version of OECD 202.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- adopted September 19, 1984
- Deviations:
- yes
- Remarks:
- EC50 after 24 hours is being reported
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dilithium disodium (5,5'-diamino-(μ-4,4'-dihydroxy-1:2-κ-2,O4,O4',-3,3'-[3,3'-dihydroxy-1:2-κ-2-O3,O3'-biphenyl-4,4'-ylenebisazo-1:2-(N3,N4-η:N3',N4'-η)]-dinaphthalene-2,7-disulfonato(8)))dicuprate(2-)
- EC Number:
- 407-230-4
- EC Name:
- Dilithium disodium (5,5'-diamino-(μ-4,4'-dihydroxy-1:2-κ-2,O4,O4',-3,3'-[3,3'-dihydroxy-1:2-κ-2-O3,O3'-biphenyl-4,4'-ylenebisazo-1:2-(N3,N4-η:N3',N4'-η)]-dinaphthalene-2,7-disulfonato(8)))dicuprate(2-)
- Cas Number:
- 126637-70-5
- Molecular formula:
- Hill formula: C32 H16 Cu2 Li2 N6 Na2 O16 S4 CAS formula: C32 H16 Cu2 N6 O16 S4. 2Li 2Na
- IUPAC Name:
- dicopper(2+) dilithium(1+) disodium 4,4'-bis[2-(8-amino-1-oxido-3,6-disulfonatonaphthalen-2-yl)diazen-1-yl]-[1,1'-biphenyl]-3,3'-bis(olate)
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- according to guideline
Test solutions
- Vehicle:
- yes
Test organisms
- Test organisms (species):
- Daphnia magna
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Remarks on exposure duration:
- appropriate testing time for the OCED 202 version at that time
- Post exposure observation period:
- not applicable
Test conditions
- Hardness:
- 11.8 °DH
- Test temperature:
- 18 - 22 °C
- pH:
- DSW: pH 8.2 1/- 0.2 after aeration
test batches: pH 8.2 - 8.9 - Dissolved oxygen:
- 9.1 - 11.5 mg/l
- Salinity:
- Not being reported as such, however the detailed mineral composition of the used so-called "Dutch standard water" (DSW) is given in the original study report.
- Nominal and measured concentrations:
- Measured concentration varied between 93 and 106 % of the nominal concentrations of the test item (see study on acute fish toxicity conducted in parallel by Boger 1990).
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 ml , all-glass
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass, 250 ml, 150 ml, 100 ml
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): no flow-thru design applied
- Renewal rate of test solution (frequency/flow rate): not applicable
- No. of organisms per vessel: 10 per 100 ml medium
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: not reported
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 289 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- including mortality
- Remarks on result:
- other: 95% CL: 215 - 530 mg/l
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 18 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks:
- including mortality
- Reported statistics and error estimates:
- The EC5O-value at 24 of exposure was calculated from the probits of the percentages of affected daphnia and the logarithms of the corresponding nominal concentrations using the maximum likelihood estimation method (Finney, D.J., 1971: Probit analysis, Cambridge University Press, Cambridge, U.K., 3rd edition).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 24h-EC50 of the substance was 289 mg/l with 95% confidence interval ranging from 215 to 530 mg/l. The No Observed Effect Concentration for the registered substance with respect to mobility of Daphnia magna was found to be 18 mg/l after 24 hours of exposure.
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