Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 217-617-8 | CAS number: 1912-24-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 16 May 1995 to 14 June 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study is well detaled and results fully described. The study doesn't follow the EU methods however it refers to EPA method.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: Toxic Substance Control act No. 139 of 21/12/94 by the Istituto Brasilero do Meio Ambiente e dos Recursos Naturais Renovaveis - IBAMA, Brazil.
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: EPA.U.S. Environmental Protection Agency. Aerobic aquatic biodegradation. Federal Register (USA),50 (188):39277-80, 1985.
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- other: LARSON, R.J. Estimation of biodegradation potential of xenobiotic organic chemicals. Appl. Environ. Microbial.,38:11531161, 1979.
- Deviations:
- not specified
- GLP compliance:
- no
Test material
- Reference substance name:
- Atrazine
- EC Number:
- 217-617-8
- EC Name:
- Atrazine
- Cas Number:
- 1912-24-9
- Molecular formula:
- C8H14ClN5
- IUPAC Name:
- 6-chloro-N2-ethyl-N4-(propan-2-yl)-1,3,5-triazine-2,4-diamine
- Details on test material:
- Material tested: Atrazina Tecnica OXON
Common name: Atrazine
Chemical name: 2-chloro-4-ethylamine-6-isopropylamine-1,3,5-triazine
Lot: M-353
Molecular weight: 215.7 g/mol
Declared purity: 94.7%
Analysed purity (BIOAGRI): 94.2%
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- Source of inocolum:
A mixture of microorganisms was collected in the Piracicaba river at a site with a high concentration of domestic raw sewage.
Inocolum preparation:
One liter of that water was centrifugated, the supernatant was discarded and the pellet was resuspended in nutrient solution in order to inoculate the flasks used in the assay. The number of inoculated microorganisms was 5.2 x 10^6 viable cells/ml. The enumeration of the microorganisms was carried out using the direct plate counting in Petri dishes with nutrient agar (NA). - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- other: carbon from the real concentration of the product
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- The assay was conducted to determine the the metabolization of Atrazina Tecnica OXON in a mineral nutrient solution by a mixture of environmental microrganisms. The study lenght was 28 days in an incubation chamber at a controlled temperature (ca 25°C). Two replicates of four treatments: (a - test substance, b - glucose, c - inhibition and d - check) were used containing 3 liters of nutrient solution inoculated with microorganisms. the CO2 evolved from all the treatments was captured in Ba(OH)2 and determined by HCl titration.
Reference substance
- Reference substance:
- other: glucose
Results and discussion
- Preliminary study:
- Preliminary study not performed.
- Test performance:
- The assay was carried out according to the following system:
six plastic bottle were filled with 700 ml 6N NaOH and connected in series using rubber tubing, to a pressurized free-CO2 air source.
the assay was carried out in completly darkness with 2 replicates in 4 L vessels, containing 3 liters of basal culture and adjusted to the test temperature (ca 25°C) in a flow-through system during 28 days.
A. Check - basal culture medium
B. Standard product - glucose at the concentration of 20 mg carbon per liter of solution.
C. Test substance - the test substance was Atrazina tecnica OXON at a concentration of 10.0 mg carbon from the real concentration of the product being tested, per liter of solution.
D. Inhibition assay - glucose was used as carbon source (20.0 mg C7L) and the test substance Atrazina tecnica OXON (10.0 mg C/L).
The solution was inoculated with a small number of microorganisms from a mixed polulation and aerated with CO2 - free air for 24 hours, to purge the system of carbon dioxide at ca 25°C, in dark condition.
After inoculation, 71.28 mg of test compound were added into the all treatments. Then 3 CO2 absorber bottles were filled with 100 ml 0.025N Ba(OH)2 and connected in series to the exit air line of each test treatments.
The biodegradation was analysed by the amounts of CO2 evolved and captured in Ba(OH)2 from all the treatments during all test period trhough HCl titration.
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0.4
- Sampling time:
- 1 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 9.86
- Sampling time:
- 28 d
Any other information on results incl. tables
Percentage of CO2 evolved from glucose, Atrazina Tecnica OXON and inhibition solution, as related to the theoretically expected CO2, during 28 days incubation period:
CO2(mg) |
||||||
Days |
Glucose |
Atrazina Tec. OXON |
Inhibition |
|||
01 |
16.26 |
31.21 |
0.80 |
0 |
8.17 |
6.87 |
02 |
37.99 |
35.94 |
1.65 |
0.15 |
22.45 |
22.12 |
03 |
54.02 |
51.22 |
1.70 |
0.20 |
30.10 |
28.40 |
04 |
62.60 |
60.35 |
1.75 |
1.65 |
34.26 |
33.16 |
05 |
68.31 |
67.41 |
4.16 |
3.65 |
37.32 |
38.52 |
08 |
72.23 |
71.83 |
6.91 |
6.80 |
45.31 |
45.41 |
12 |
75.13 |
74.13 |
7.01 |
7.40 |
51.01 |
51.71 |
16 |
76.06 |
75.56 |
7.16 |
8.55 |
55.96 |
56.89 |
20 |
78.34 |
76.93 |
8.81 |
10.70 |
60.61 |
61.14 |
24 |
79.81 |
78.51 |
8.86 |
10.75 |
65.23 |
64.46 |
28 |
80.29 |
79.14 |
8.91 |
10.80 |
65.64 |
68.34 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- The test was considered valid because the CO2 evolved from the standard solution was greater than 70% within 10 days interval after the date when the CO2 evolved reached 10%.
- Interpretation of results:
- other: under test contidions any biodegradation was observed
- Conclusions:
- According to the results, the product Atrazina Tecnica OXON had low evolution of carbon dioxide through out incubation period. This chemical had a total of 9.86% CO2 evolved from total carbon by the end of the assay. The glucose control had 79.71% and the inhibition test had 66.99% indicating Atrazina Tecnica OXON not toxic to microorganisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.