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EC number: 939-685-4 | CAS number: 1474044-71-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- Adopted March 23, 2006; Annex 5 corrected 28 July 2011
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Name of test material: 1-Propanaminium, 2-hydroxy-N-(2-hydroxypropyl)-N,N-dimethyl-, esters with fatty acids, C18 unsatd., Me-sulfates (salts)
- Physical state: liquid
- Analytical purity: 100% - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- Preparation of test solutions started with a loading rate of 10 mg/L applying 2 hours of magnetic stirring to reach maximum solubility of the test substance in the test medium. The lower test concentrations were prepared by subsequent dilutions of the 10 mg/L concentration in test medium. All test vessels were pre-incubated for 30 minutes with the respective test solution before the start of the test.
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- Strain: Pseudokirchneriella subcapitata, NIVA CHL 1
- Source (laboratory, culture collection): in-house laboratory culture.
- Age of inoculum (at test initiation): 4 d (final test), 3 d (range-finding test)
- Method of cultivation: stock cultures were started by inoculating growth medium with algal cells from a pure culture on agar. The suspensions were continuously aerated and exposed to light in a climate room at a temperature of 21-24°C
ACCLIMATION
- Culturing media and conditions (same as test or not): same - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 21.5 - 22.5°C
- pH:
- 8.1-8.3
- Nominal and measured concentrations:
- nominal: 0, 0.10, 0.32, 1.0, 3.2 and 10 mg/L + 4 mg humic acid per litre
measured (TWA): 0.042, 0.14, 0.39, 1.3 and 1.7 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL, all-glass, containing 50 mL of test solution
- Initial cells density: 1E04 cells/mL
- Control end cells density: 66.1E04 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M2; according to the OECD 201 Guideline, formulated using Milli-RO water
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous light
- Light intensity and quality: TLD-lamps type ‘Cool-white’ 30 Watt, light intensity 94 to 107 E/m²/s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometer
- Other: At the end of the test microscopic observations were performed on the lowest test concentration (range-finding test) or on the test concentration closest to the EC50 (both final tests) and 1 lower concentration (final test with humic acid) to observe for any abnormal appearance of the algae
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2 (sqrt(10))
- Range finding study
- Test concentrations: nominal 0, 0.010, 0.10, 1.0 and 10 mg/L, without humic acid
- Results used to determine the conditions for the definitive study: ECr50 between nominal concentrations of 0.10 and 1.0 mg/L, EyC50 between nominal concentrations of 0.010 and 0.10 mg/L (without humic acid)
At the start of the test, the actual test concentrations were in agreement with nominal, i.e. 86-87% of initial. These concentrations decreased to 2-39% of initial after 24 hours of exposure and further to 1-13% of initial et the end of the test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.54 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% ci 0.10-2.9 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% ci 0.24-6.0 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.49 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% ci 0.22-1.0 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.95 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- act. ingr.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% ci 0.44-2.1 mg/L
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): Microscopic observations revealed no abnormalities
After 48 hours of exposure a brown layer was observed sticking to the glass just above the medium surface at an average concentration of 1.7 mg/L. After 72 hours of exposure a light floating layer and precipitate was observed at an average concentration of 0.39 mg/L; a floating layer, precipitate and a brown layer was observed sticking to the glass just above the medium surface at average exposure concentrations of 1.3 and 1.7 mg/L. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- ErC50 = 0.97 mg/L, 95% confidence interval 0.62 to 1.5 mg/L; historical ranges for growth rate reduction lie between 0.82 and 2.3 mg/L - Reported statistics and error estimates:
- For determination of the NOEC and the EC50 the approaches recommended in the OECD guideline 201 were used. An effect was considered to be significant if statistical analysis of the data obtained for the test concentrations compared with those obtained in the negative control revealed significant reduction of growth rate or inhibition of yield (ANOVA, Bonferroni t-Test, TOXSTAT Release 3.5, 1996, D.D. Gulley, A.M. Boelter, H.L. Bergman). Additionally, the EC10 was determined to meet the recommendations as put down in "A Review of Statistical Data Analysis and Experimental Design in OECD Aquatic Toxicology Test Guidelines" by S. Pack, August 1993. Calculation of the EC50 and EC10 values was based on log-linear regression analysis of the percentages of growth rate reduction and the percentages of yield inhibition versus the logarithms of the corresponding average exposure concentrations of the test substance.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC, EC10 and EC50 of MDIPA Esterquat C18 unsatd. to algae in the presence of humic acid based on growth rate were 0.39, 0.54 (95% c.i. 0.10-2.9 mg/L) and 1.2 mg/L (95% c.i. 0.24-6.0 mg/L), respectively.
The NOEC, EC10 and EC50 based on based on yield were 0.39, 0.49 (95% c.i. 0.22-1.0 mg/L) and 0.95 mg/L (95% c.i. 0.44-2.1 mg/L), respectively. - Executive summary:
In a 72 hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata, strain NIVA CHL 1 were exposed to MDIPA Esterquat C18 unsatd. at nominal concentrations of 0 (control), 0.10, 0.32, 1.0, 3.2 and 10 mg/L, corresponding to measured (TWA) 0.042, 0.14, 0.39, 1.3 and 1.7 mg/L in the presence of 4 mg/L humic acid under static conditions in accordance with OECD guideline 201 (adopted March 23, 2006; Annex 5 corrected 28 July 2011).
Microscopic observations revealed no abnormalities.
The NOEC, EC10 and EC50 based on growth rate were 0.39, 0.54 (95% c.i. 0.10-2.9 mg/L) and 1.2 mg/L (95% c.i. 0.24-6.0 mg/L), respectively.
The NOEC, EC10 and EC50 based on based on yield were 0.39, 0.49 (95% c.i. 0.22-1.0 mg/L) and 0.95 mg/L (95% c.i. 0.44-2.1 mg/L), respectively.
All effect levels are given based onmeasured (TWA) concentrations.
Results Synopsis
Test Organism:Pseudokirchneriella subcapitata, strain NIVA CHL 1
Test Type: Static
72 hr ErC10: 0.54 mg a.i./L 95% c.i. 0.10-2.9 mg/L
72 hr ErC50: 1.2 mg a.i./L 95% c.i. 0.24-6.0 mg/L
72 hr EyC10: 0.49 mg a.i./L 95% c.i. 0.22-1.0 mg/L
72 hr EyC50: 0.95 mg a.i./L 95% c.i. 0.44-2.1 mg/L
72 hr NOEC: 0.39 mg a.i./L
Endpoint(s) Effected: growth rate, yield
Reference
At the start of the test, the actual test concentrations were in agreement with nominal, i.e. 107-115% of initial. These concentrations decreased to 15-57% of initial after 24 hours of exposure and further to 1-6% of initial et the end of the test. The average exposure concentrations were calculated to be: 0.042, 0.14, 0.39, 1.3 and 1.7 mg/L.
At the start of the test measured T(D)OC concentrations ranged between 1.7 and 2.3 mg/L for all concentrations up to and including 1.0 mg/L. These concentrations remained stable during the test. Measured T(D)OC at 3.2 and 10 mg/L were 3.2 and 6.4 mg/L, respectively, at the start of the test and decreased to 2.5 and 1.6 mg/L, respectively, at the end of the test.
Mean cell densities (x 1E04 cells/mL) during the final test with humic acid
Average conc. |
Exposure time (hours) |
|||
(mg/L) |
0 |
24 |
48 |
72 |
Humic acis-control |
1.0 |
2.5 |
14.6 |
66.1 |
0.0421 |
1.0 |
2.6 |
12.9 |
73.7 |
0.141 |
1.0 |
2.0 |
11.9 |
72.6 |
0.391 |
1.0 |
3.1 |
11.1 |
66.2 |
1.32 |
1.0 |
2.4 |
6.6 |
33.4 |
1.73 |
1.0 |
1.0 |
1.0 |
1.0 |
1Clear yellow solution
2Slightly hazy yellow solution
3Hazy yellow solution
Percentage reduction of growth rate (total test period) and percentage inhibition of yield during the final test with humic acid
Average conc. |
Mean growth rate |
Yield (0-72 h) |
||
(mg/L) |
µ (0-72 h) |
Reduction (%) |
x104cells/mL |
Inhibition (%) |
Humic acid-control |
0.05815 |
65.13 |
||
0.042 |
0.05971 |
-2.7 |
72.67 |
-11.6 |
0.14 |
0.05951 |
-2.3 |
71.58 |
-9.9 |
0.39 |
0.05809 |
0.1 |
65.17 |
-0.1 |
1.3 |
0.04870 |
16.3 |
32.42 |
50.2 |
1.7 |
0.00000 |
100.0 |
0.00 |
100.0 |
Description of key information
72 h NOEC (growth rate) = 0.39 mg/mL, 72 h ErC10 = 0.54 mg/L (95% c.i. 0.10-2.9 mg/L), 72 h ErC50 = 1.2 mg/L (95% c.i. 0.24-6.0 mg/L);
72 h NOEC (biomass) = 0.39 mg a.i./mL, 72 h EyC10 = 0.49 mg/L (95% c.i. 0.22-1.0 mg/L), 72 h EyC50 = 0.95 mg/L (95% c.i. 0.44-2.1 mg/L)
(Pseudokirchneriella subcapitata); OECD guideline 201, static, GLP, RL1; in the presence of 4 mg/L humic acid
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.2 mg/L
- EC10 or NOEC for freshwater algae:
- 0.54 mg/L
Additional information
In a 72 hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata, strain NIVA CHL 1 were exposed to MDIPA Esterquat C18 unsatd. at nominal concentrations of 0 (control), 0.10, 0.32, 1.0, 3.2 and 10 mg/L, corresponding to measured (TWA) concentrations of 0.042, 0.14, 0.39, 1.3 and 1.7 mg/L in the presence of 4 mg/L humic acid under static conditions in accordance with OECD guideline 201 (adopted March 23, 2006; Annex 5 corrected 28 July 2011).
Microscopic observations revealed no abnormalities.
The NOEC, EC10 and EC50 based on growth rate were 0.39, 0.54 (95% c.i. 0.10-2.9 mg/L) and 1.2 mg/L (95% c.i. 0.24-6.0 mg/L), respectively.
The NOEC, EC10 and EC50 based on based on yield were 0.39, 0.49 (95% c.i. 0.22-1.0 mg/L) and 0.95 mg/L (95% c.i. 0.44-2.1 mg/L), respectively.
All effect levels are given based on measured (TWA) concentrations.
Similar results were obtained with the closely related read-across substances MDEA-Esterquat C16-18 and C18 unsatd. and MDEA-Esterquat C16-18 and C18 unsatd.:
As a weight of evidence data are available from a substance with the same structure as MDEA-Esterquat C16-18 and C18 unsatd., only the distribution of fatty acid moiety is shifted to higher amount of unsaturated C18' (Iodine value 47 instead of 25). From a higher amount of unsaturated C18 a higher toxicity is to be expected. In this 72 -h algal study conducted according to OECD TG 201, Scenedesmus subspicatus (new name Desmodesmus subspicatus) were exposed to the similar substance ethanaminium, 2-hydroxy-N-(-2-hydroxyethyl)-N,N-dimethyl-, esters with C16-18 and C18 unsatd. fatty acids, chlorides (Iodine value of 47 instead of 25, the highest value for the submitted substance) at measured concentrations of 0, 1.5, 2.8, 6.6, 16, 29 and 64 mg/L under static conditions. The 72 -h EC50 based on biomass is 3.2 mg/L with 95% confidence limits of 2.7 - 3.6 and the 72 -h EC50 based on growth rate is 6.3 mg/L with 95% confidence limits of <1.5 - >64. The 72 -hr NOEC based on growth rate and biomass is 1.5 mg/L (measured concentrations each).
In a 96 h algal toxicity study conducted according to a modification of EPA OTS 797.1050 (Algal Toxicity, Tiers I and II), Selenastrum capricornutum was exposed MDEA-Esterquat C16-18 and C18 unsatd. at nominal concentrations of 0, 2, 4, 8, 16 and 32 mg/L under static conditions. The 96 hr ErC50 value was 2.9 mg/L ( 95% confidence limit <2.0 - 4.2 mg/L; nominal).
This toxicity study is classified as not reliable and does not satisfy the guideline requirements for aquatic toxicity to algae (validity Klimisch 3). Deviations were: no analytical determination of test substance, river water used instead of algal media, and cell counts only done on controls at 0 h timepoint.
In a 72 hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata, strain NIVA CHL 1 were exposed to MDIPA-Esterquat C16-18 and C18 unsatd. at nominal concentrations of 0 (control), 0.25, 0.80, 2.5, 8.0 and 25 mg/L, corresponding to measured (TWA) 0.29, 0.91, 2.7, 6.2 and 25 mg/L in the presence of 4 mg/L humic (<2 mg/L DOC) acid under static conditions in accordance with OECD guideline 201 (adopted March 23, 2006; Annex 5 corrected 28 July 2011). Microscopic observations revealed no abnormalities. The NOEC, EC10 and EC50 based on growth rate were 2.7, 3.2 (95% c.i. 2.5-4.1 mg/L) and 8.1 mg/L (95% c.i. 6.4-10 mg/L), respectively. All effect levels are given based on measured (TWA) concentrations.
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