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Diss Factsheets
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EC number: 201-369-2 | CAS number: 81-68-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 December, 1993 to 14 December, 1993
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- (Deionised water instead of distilled water used for preparation of stock solution; preparation of the inoculum: higher sludge weight after the washing procedure (sludge concentration in the test according to the guidelines); 4 instead of 5 test conc)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Test material: FAT 36034/E
Batch No.: 9300102
Expiry date: July, 1998
Purity: 93 %
Solubility: in water <0.1 g/L
Colour: red
Storage: at room temperature - Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- Stock solution: The concentration of the stock "solution" is 0.5 g/L. It was prepared using a 10 minutes ultrasonic treatment for homogenization.
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Organism: Activated sludge of a communal sewage treatment plant (ARA-Birsig Therwil)
Sludge pretreatment: 3 times washing with tap water and addition of synthetic sewage (a day before the main test) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21 ⁰C
- pH:
- 7.7-7.9
- Nominal and measured concentrations:
- Nominal concentrations: 10, 32, 100 and 320 mg/L
- Details on test conditions:
- Sludge dry weight: 1.44 g/L
Test volume: 100 mL
Estimation technique: O2-consumption, measured with an oxygen sensitive electrode system: OXI DIGI 530 (SAV: 00 109)
Aeration: 0.1 - 0.2 L/min by Pasteur-pipette
pH of sludge: 7.8 before the test beginning - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 314 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The % inhibitions observed at 0, 0, 0.57 and 0 % at 10, 32, 100 and 320 mg/L, respectively.
- Results with reference substance (positive control):
- The IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 13 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The IC50 of the test substance was determined to be > 320 mg/L (i.e. > 298 mg a.i./L).
- Executive summary:
A study was conducted to assess the inhibitory effect of the test substance (of ca. 93 % purity) on the oxygen consumption rate of aerobic microorganisms (activated sludge) according to OECD Guideline 209 in compliance with GLP.
Activated sludge of a communal sewage treatment plant was exposed to the test substance (10, 32, 100 and 320 mg/L) for 3 h and analyzed for oxygen consumption rate. The % inhibitions observed at 0, 0, 0.57 and 0 % at 10, 32, 100 and 320 mg/L, respectively. Hence, the IC50 of the test substance was determined to be >320 mg/L (i.e. >298 mg a.i./L).
Reference
Results:
Control | pH after 3h | 1) O2-consumption (in cm) | 2) time (in cm) | mg O2 / min/L | Legend: C =Control; 1) 2 cm=0.8 mg/L O2; 2) 2 cm= 1 minute |
C1 C2 | 8.0 7.7 | 4.3 4.4 | 3.8 4.2 | 0.91 0.84 | ΦC 0.875 C1/C2: 8.33 % C2/C2: 7.69 % |
Test substance(mg/L) | pH after 3h | 1) O2-consumption (in cm) | 2) time (in cm) | mg 0 2 pro minute/L | % of control | % inhibition |
320 | 7.7 | 4.9 | 4.3 | 0.91 | 104.00 | 0.00 |
100 | 7.9 | 4.7 | 4.3 | 0.87 | 99.43 | 0.57 |
32 | 7.8 | 5.3 | 4.7 | 0.90 | 102.86 | 0.00 |
10 | 7.8 | 4.4 | 4.0 | 0.88 | 100.57 | 0.00 |
3,5- Dichlorophenol (mg/L) | pH after 3h | 1) O2-consumption (in cm) | 2) time (in cm) | mg 0 2 pro minute/L | % of control | % inhibition |
32 | 7.9 | 1.0 | 5.9 | 0.14 | 16.00 | 84.00 |
10 | 7.7 | 3.0 | 4.6 | 0.52 | 59.43 | 40.57 |
3.2 | 7.7 | 4.0 | 4.1 | 0.78 | 89.14 | 10.86 |
Description of key information
A study was conducted to assess the inhibitory effect of the test substance (of ca. 93 % purity) on the oxygen consumption rate of aerobic microorganisms (activated sludge) according to OECD Guideline 209 in compliance with GLP. Activated sludge of a communal sewage treatment plant was exposed to the test substance (10, 32, 100 and 320 mg/L) for 3 h and analyzed for oxygen consumption rate. 3,5-dichlorophenol was used as reference material. Under the test conditions, the IC50 of the test substance was determined to be > 320 mg/L (i.e. > 298 mg a.i./L).
This outcome was further supported in studies with FAT 36034/A and FAT 36034/B, where the IC50 was observed to be >300 mg/L (nominal; test material).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 320 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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