4-Methylsulfonyl-2-nitro benzoic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

August 10, 2009 - August 14, 2009

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OSPAR Commission 2006: Protocols on Methods for the Testing of Chemicals Used in the Offshore Industry (Part B was amended in 2006 (OSPAR summary record 06/23/1, §9.7))

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

4-methanesulfonyl-2-nitrobenzoic acid

EC Number:

601-017-1

Cas Number:

110964-79-9

Molecular formula:

C8 H7 N O6 S

IUPAC Name:

4-methanesulfonyl-2-nitrobenzoic acid

Details on test material:

Substance delivered by Cheminova to DHI August 7, 2009. Stored at room temperature.

Test organisms

Test organisms (species):

other: Scophthalmus maximus

Details on test organisms:

Animals were bought from the commercial producer – Maximus at Thisted, Denmark.

Study design

Water media type:

saltwater

Total exposure duration:

96 h

Test conditions

Test temperature:

15.9 +- 0.1 C

pH:

8.0

Dissolved oxygen:

> 70%

Salinity:

32 o/oo

Nominal and measured concentrations:

Nominal: 50; 100; 200; 500 and 1000 mg/l

Details on test conditions:

Light/dark period at 16:8 hours

Reference substance (positive control):

yes

Remarks:

3,5-dichlorphenol (3,5-DCP)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No effects were seen

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No effects were seen

Details on results:

The lethality for the control group was at the end of the test less than 10%.
The oxygen saturation for all aquaria were > 60% during the whole test period.
The fish were a little smaller than recommended by OSPAR, since their average length was 3.38 +- 0.30 cm. The average weight was 1.01 +- 0.24 g and in that way the requirement about maximally 1 gram fish per litre test solution is kept.

Results with reference substance (positive control):

The reference substance was tested at 0.3; 0.6; and 1.2 mg 3,5-DCP. The toxicity was 0% lethality at 0.6 mg/l and 100% at 1.2 mg/l. That is below the recommended 20-80% lethality at 0.6 mg/l, but since the lethality was 100% at 1.2 mg/l the sensitivity of the fish is assessed to be normal.

Applicant's summary and conclusion

Conclusions:

Since no effects were seen, the value of both 10% lethality (LC19) and 50% lethality (LC50) are estimated to be > 1000 mg/l.