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EC number: 811-852-5 | CAS number: 1792219-00-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-12-07 - 2017-01-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-{[1,1'-biphenyl]-4-yl}-N-[2-(9,9-diphenyl-9H-fluoren-4-yl)phenyl]-9,9-dimethyl-9H-fluoren-2-amine
- EC Number:
- 811-852-5
- Cas Number:
- 1792219-00-1
- Molecular formula:
- C58H43N
- IUPAC Name:
- N-{[1,1'-biphenyl]-4-yl}-N-[2-(9,9-diphenyl-9H-fluoren-4-yl)phenyl]-9,9-dimethyl-9H-fluoren-2-amine
- Test material form:
- solid: particulate/powder
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, 31137 Hildesheim, Germany
- Pretreatment: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration for 2 hours. Thereafter, the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and maintained in an aerobic condition by aeration with CO2-free air for 7 days. 10 mL/L were used to initiate inoculation.
- Initial cell/biomass concentration: Approx. 10E+7 – 10E+8 CFU/L - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 18 mg/L
- Based on:
- test mat.
- Initial conc.:
- 2.89 other: mg O2/mg
- Based on:
- ThOD
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium according to OECD 301 F
- Test temperature: Nominal: 20 - 24 ± 1 °C; Actual: 20.5 - 21.5 °C
- Continuous darkness: Dark, in an incubator
- pH: 7.63 – 7.89
TEST SYSTEM
- Culturing apparatus: brown glass bottles
- Number of culture flasks/concentration: two for the inoculum control (C1, C2), one for the functional control (R1), two for the test item (P1, P2), one for the toxicity control (T 1)
- Measuring equipment: A rubber sleeve with soda lime was hung into the opening of the bottles to absorb evolved CO2. The bottles were closed with OxiTop® measuring heads and the measuring system was activated.
SAMPLING
- Sampling frequency: The measuring were done at 360 measuring points (every 112 minutes).
CONTROL AND BLANK SYSTEM
- Inoculum blank: The inoculated test medium, consisting of the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were prepared in a measuring flask. 250 ml of this solution will be filled in the brown glass bottles of the inoculum control, using a 250 mL measuring flask.
- Toxicity control: For the toxicity control, the test item was weighed out and directly transferred into the test vessel with 250 ml of the reference item solution, using a 250 mL measuring flask.
- Functional control: The reference item was weighed out and transferred into a measuring flask, the required volumes of mineral medium stock solutions, ultrapure water and inoculum, were added. 250 mL of this solution were filled in the brown glass bottle of the functional control, using a 250 mL measuring flask.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Details on results:
- In the toxicity control the biodegradation achieved 54% after 14 days. After 28 days the biodegradation came to 56%. The validity criterion that the test item should not inhibit the biodegradation of the reference item was fulfilled.
BOD5 / COD results
- Results with reference substance:
- The pass level for ready biodegradation (> 60% degradation) was reached on day 4. The validity criterion that the degradation should be > 60% after 14 d was fulfilled.
Stages of Biodegradation: The adaptation phase changed to degradation phase within 2 days (degradation > 10%) and the biodegradation came to a maximum of 80% on day 19.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item was not readily biodegradable within the 28 day period of the study in the Manometric Respiratory Test according to OECD Guideline 301F.
- Executive summary:
The ready biodegradability of the test item was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days according to OECD Guideline 301 F at the test facility. The test item concentration selected as appropriate was 18 mg/L, corresponding to a ThOD of 52.0 mg 02/L (53.5 mg 02/L with nitrification) per test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation rate is therefore expressed as the percentage BOD (biological oxygen demand) and was calculated for each study day.
The mean oxygen depletion in the inoculum control was 9.0 mg 02/L on day 28. In order to check the activity of the test system sodium benzoate was used as functional control.
The pass level > 60% was reached on day 4. The biodegradation reached a maximum of 80% degradation on day 19. In the toxicity control containing both test and reference item 54% degradation occurred within 14 days. After 28 days the biodegradation came to 56%. The degradation of the reference item was not inhibited by the test item.
Both test item replicates did not reach the 10% level (beginning of biodegradation) within the 28 day period of the study. The mean biodegradation on day 28 was 1 %.
The validity criteria of the guideline are fulfilled.
The test item is classified as not readily biodegradable within the 28 day period of the study.
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