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Reaction mass of Cobaltate(1-), bis[6-(amino-κN)-5-[2-[2-(hydroxy-κO)-4-nitrophenyl]diazenyl-κN1]-N-methyl-2-naphthalenesulfonamidato(2-)]-, sodium (1:1) and disodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)][6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]naphthalene-2-sulphonato(3-)]cobaltate(2-)
EC number: 943-062-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental starting date: 29 November 1994 and Experimental termination date: 01 December 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- limit test: 3 concentrations tested.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Code number: FAT 20036/F
Batch-No: 1
Purity: ca. 97.7 %
Appearance: Solid
Solubility: 80 mg/L (in water)
Storage: room temperature
Expiration date: 07/99 - Analytical monitoring:
- yes
- Details on sampling:
- All test medium samples for the analytical determinations were taken from the approximate center of the test vessels (without mixing the medium) and kept at -18 °C to -25 °C until analysis.
The concentrations of the test substance at the beginning and at the end of the test were analysed by the PE Analytical Services K, Textile Dyes Division. - Vehicle:
- yes
- Details on test solutions:
- The nominal test concentrations were chosen as 45, 100 and 220 mg test article/I.
For the preparation of the test concentrations only the supernatant of the stock solution was taken.
All test concentrations contained the vehicle Tween 80 (Polyoxyethylensorbitanmonooleat) in a concentration of 5 ppm. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The organism used in this test system is the species Daphnia magna. The Daphnia were bred in the laboratories of the Product Ecology TF, Textile Dyes Division under standardised conditions.
*Breeding:
- Cultures of Daphnia magna were held in glass vessels containing approx. 3.0 litre of reconstituted water at 18 - 22°C. Water was renewed partially three times/week. Daphnia's were fed with a suspension of green algae (Scenedesmus subspicatus) supplemented by a TETRAMIN-extract in such quantites that the food was consumed within 24h.
*Pretreatment:
- 24 hours before the start of the exposure reproductive, Daphnia were separated from the young Daphnia by sieving all individuals through a 1000 µm sieve.
For each test concentration, 20 Daphnia divided in two groups of 10 animals were used. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- None
- Post exposure observation period:
- Not available
- Hardness:
- 197 mg/l CaCO3
- Test temperature:
- 21 ± 1 °C
- pH:
- 7.8 - 8.2
- Dissolved oxygen:
- Not available
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- The nominal test concentrations were chosen as 45, 100 and 220 mg test article/L.
For the preparation of the test concentrations, only the supernatant of the stock solution was used. - Details on test conditions:
- - Test procedure: static
- Duration: 48 hours
- 12 hours light/12 hours dark.
The Daphnia were exposed to the test substance in 250 mL beakers containing 100-200 mL test medium with 10 test animals per beaker. All tests were carried out in duplicate. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 131 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 131 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 131 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 131 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 131 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 131 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations. Therefore, the NOEC and EC0 value after 48 hours were determined to be 131 mg/l, the EC50 and the EC100 value could not be calculated, but are clearly greater than 131 mg/L.
- Results with reference substance (positive control):
- The quality of the Daphnia will be checked at least every 3 months by determining the EC50 value (24 h) for potassium dichromate (range: 0.8 -1.5 mg/l). Last reference control at September 09, 1994 ; EC50 : 1.16 mg/l.
- Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations. Therefore, the NOEC and EC0 value after 48 hours were determined to be 131 mg/l, the EC50 and the EC 100 value could not be calculated, but are clearly greater than 131 mg/l.
- Executive summary:
The acute toxicity of FAT 20036/F to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adopted 4 April 1984 ( issued 1993 ) and EU-Guideline 92/69, EEC Nr. L 383 A, C. 2, (29th Dec. 1992). The study was performed under Good Laboratory Practice (GLP) conditions.
The test concentrations were prepared from a 1.0 g/L stock solution. Although the test substance is soluble in pure (deionised) water (about 80 g/l, most of the test substance in the stock solution settled down and build a sediment at the bottom of the flask. This effect was possibly caused by a reaction of the test substance with the water hardness compounds in the test water. For the preparation of the test concentrations only the supernatant of the stock solution was taken ( limit of the solubility).
The nominal concentrations tested were 45, 100 and 220 mg test article/l, a vehicle and a blank control without any addtions. In the nominal test concentrations of 100 and 220 mg/l a sediment at the bottom of the test vessel was observed after 24 hours.
Because of the low solubility of the test article in the test media, the actual test concentrations in the analysed test media were in the range of 45 - 74 % of the nominal values. Therefore, the reported results are related to the actual mean values of the analysed test concentrations of the test article measured at the beginning and at the end of the test.
No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations. Therefore, the NOEC and EC0 value after 48 hours were determined to be 131 mg/l, the EC50 and the EC 100 value could not be calculated, but are clearly greater than 131 mg/l.
Results (24hrs)
EC0: 131 mg/L
EC50: >131 mg/L
EC100: > 131 mg/L
Results (48hrs)
EC0: 131 mg/L
EC50 : >131 mg/L
EC100 > 131 mg/L
NOEC 131 mg/L
Conclusion:
FAT 20036/F has been tested up to the limit of solubility in the test water. According to Directive 93/21/EEC ( adaptation to Directive 67/548/EEC ) the ecotoxicological classification of FAT 20036/F is not toxic to Daphnia magna after 48 hours.
Reference
The test concentrations were prepared from a 1.0 g/l stock solution. Although, the test substance is soluble in pure (deionised) water (about 80 g/l), a part of the test substance in the stock solution settled down and build a sediment at the bottom of the flask. This effect was possibly caused by a reaction of the test substance with the water hardness compounds in the test water. For the preparation of the test concentrations only the supernatant of the stock solution was taken ( limit of the solubility ).
The nominal concentrations tested were 45, 100 and 220 mg test article/L, a blank and a vehicle control without any test material. In the test concentrations of 100 and 220 mg/l, nominal a sediment at the bottom of the test vessel was observed after 24 hours.
Because of the low solubility of the test article, the actual test conentrations in the analysed test media were below 45 - 74 % of the nominal values. Therefore, the reported results are related to the actual mean values of the analysed test concentrations of the test article measured after beginning and at the end of the test.
No immobilization and abnormal behaviour of the Daphnia was observed in the controls and in the test concentrations. Therefore, the NOEC and EC0 value after 48 hours were determined to be 131 mg/l, the EC50 and the EC-JOO value could not be calculated, but are clearly greater than 131 mg/l.
Immobilization of Daphnia
Number of immobilized Daphnia after 24 and 48 hours
Actual cone, oftest substance(mg/l) | N° of animals | N° of response 24 h | N° of response 24 h | % immobility 24 h | % immobility 48h |
Blank | 10 | 0 | 0 | 0 | 0 |
Vehicle | 10 | 0 | 0 | 0 | 0 |
27 | 10 | 0 | 0 | 0 | 0 |
58 | 10 | 0 | 0 | 0 | 0 |
131 | 10 | 0 | 0 | 0 | 0 |
Analysis of the test substance
Analytical determination of test substance concentrations
Strat concentrations | end concentrations | ||||
Nominal conc of test substance (mg/l) | mg/l | % of nominal | mg/l | % of nominal | Actual mean conc. mg/l |
Blank | <3.0 | / | <3.0 | / | <3.0 |
Vehicle | <3.0 | / | <3.0 | / | <3.0 |
45 | 24 | 53 | 30 | 67 | 27 |
100 | 47 | 47 | 68 | 68 | 58 |
220 | 99 | 45 | 162 | 74 | 131 |
Method : Spectrophotometric Method AAK 61/94 ; SOP FO 3.31-2.3
Detection limit : 3.0 mg/l ( related to the nominal concentrations )
Measurements
The oxygen concentration, the temperature and the pH was controlled at the beginning and at the end of the test.
Start:
Oxygen: saturated
pH: 8.0 ( in the highest test concentration )
Temperature: 21 °C
End:
Nominal conc. (mg/l) | Blank | Vehicle | 45 | 100 | 220 |
Oxygen % | 99 | 99 | 98 | 97 | 95 |
pH | 7.7 | 7.8 | 7.7 | 7.7 | 7.7 |
Temperature °C | 20.5 | 20.5 | 20.5 | 20.5 | 20.5 |
ANALYTICAL DETERMINATION OF TEST ARTICLE IN DAPHNIA WATER
RESULTS
Designation of the sample: Content of test article
Test no. G 258 14: in daphnia water
Start | blank | 29.11.1994 | / | <3.0 mg/l |
end | blank | 01.12.1994 | / | <3.0 mg/l |
Start | vehicle | 29.11.1994 | / | <3.0 mg/l |
End | Vehicle | 01.12.1994 | / | <3.0 mg/l |
Start/A | 45 mg/l | 29.11.1994 | 24.4 mg/l | |
Start/B | 45 mg/l | 29.11.1994 | 23.8 mg/l | 24 mg/l |
Ende/A | 45 mg/l | 01.12.1994 | 30.2 mg/l | |
Ende/B | 45 mg/l | 01.12.1994 | 30.5 mg/l | 30 mg/l |
Start/A | 100 mg/l | 29.11.1994 | 45.9 mg/l | |
Start/B | 100 mg/l | 29.11.1994 | 47.7 mg/l | 47 mg/l |
Ende/A | 100 mg/l | 01.12.1994 | 69.3 mg/l | |
Ende/B | 100 mg/l | 01.12.1994 | 67.2 mg/l | 68 mg/l |
Start/A | 220 mg/l | 29.11.1994 | 99.8 mg/l | |
Start/B | 220 mg/l | 29.11.1994 | 98.3 mg/l | 99 mg/l |
Ende/A | 220 mg/l | 01.12.1994 | 161.2 mg/l | |
Ende/B | 220 mg/l | 01.12.1994 | 163.4 mg/l | 162 mg/l |
Description of key information
A study was conducted according to OECD guideline 202 to evaluate acute toxicity potential of FAT 20036/F to aquatic invertebrates (Daphnia magnia).
According to the results of the test, the NOEC and EC0 value after 48 hours were determined to be 131 mg/l, the EC50 and the EC 100 value could not be calculated, but are clearly greater than 131 mg/l.
Based on this data, the test substance FAT 20036/F can be considered as non-toxic to aquatic invertebrates.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 131 mg/L
Additional information
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