1-methoxy-2-propanamine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-09-04 to 1989-09-08

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP guideline study without analytical monitoring, no detailed information on the preparation of the test solutions given.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412 L9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

609-333-1

EC Number:

609-333-1

Cas Number:

37143-54-7

Molecular formula:

C4 H11 N O

IUPAC Name:

609-333-1

Details on test material:

- Analytical purity: 99.5 %
- Physical appearance: Colorless - yellowish

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution containing 0.1% was prepared.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Green algae
- Strain: SAG 86.81
- Source: Algensammlung Göttingen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

23.9 °C

pH:

7.9 - 9.76

Nominal and measured concentrations:

Nominal concentrations: 0, 10, 25, 50, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Initial cells density: 11000 to 13000 cells per mL
- Control end cells density: 286000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: According to guideline
- Intervals of water quality measurement: Test start and test end

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Biomass, growth rate
- Determination of cell concentrations: Fluorimeter

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: yes
- Test concentrations: 0.05, 0.5, 5.0, 50.0 and 500.0 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

25.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% CL: 13.7 - 46.8 mg/L

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

34.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% CL: 19.3 - 62.2 mg/L

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

28 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95% CL: 10.7 - 73.4 mg/L

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

44.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Remarks on result:

other: 95% CL: 16.5 - 120.1 mg/L

Details on results:

- Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: no

Reported statistics and error estimates:

Tallarida, R .J . Jacob, L .S . (1979) : The Dose-Response Relation in Pharmacology; S . 98 - 103, Springer-Verlag