1-methoxy-2-propanamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-10-26 to 1988-10-28

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study without analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

Method: EG-Richtlinie 79/831/EWG, Anhang V, Teil C, Methoden zur Bestimmung der Oekotoxizitaet fuer Daphnien, draft

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

609-333-1

EC Number:

609-333-1

Cas Number:

37143-54-7

Molecular formula:

C4 H11 N O

IUPAC Name:

609-333-1

Details on test material:

- Analytical purity: 99.5%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Concentration of the stock solution: 500 mg/L nominal.
- The desired test concentrations were realized by a dilution series of the stock solution.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: Daphnia magna STRAUS was originally supplied from "Institut National de Recherche Chimique Appliquée, France", and has been breeded since 1978 in the Laboratories of BASF.
- Age at study initiation (range): 2 - 24 hours old

BREEDING CONDITIONS:
- Hardness: 2.7 ±0.5 mmol/L
- Oxygen: >2 mg/L
- Type and amount of food: green algae (daily), yeast (water renewal)
- Feeding frequency: daily
- Photoperiod: 16:8 light:darkness
- Light intensity: ca. 5 µE/m²s

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.70 ± 0.5 mmol/L

Test temperature:

19 - 20 °C

pH:

8.19 - 10.25

Dissolved oxygen:

> 8 mg/L

Nominal and measured concentrations:

Test concentrations (nominal): 0, 31.25, 62.5, 125, 250 and 500 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 mL glass tubes with flat bottom, 10 mL test volume
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
Total hardness: 2.68 mmol/L
Alkalinity up to pH 4.3: 0.81 mmol/L
pH value: 8.0
Conductivity: 620 µS/cm

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

62.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

101.53 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL = 89.7 - 115.1 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None reported