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EC number: 270-151-7 | CAS number: 68411-85-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- The reliability rating is a 1 because the study followed an OECD standard guideline, which describes a procedure designed to evaluate this endpoint, and the results were reviewed for reliability and assessed as valid. The study was also conducted under GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Hydrazinecarboximidamide, 2-[(2-hydroxyphenyl)methylene]-, reaction products with 2-undecanone
- EC Number:
- 270-151-7
- EC Name:
- Hydrazinecarboximidamide, 2-[(2-hydroxyphenyl)methylene]-, reaction products with 2-undecanone
- Cas Number:
- 68411-85-8
- Molecular formula:
- C19H32N4O2
- IUPAC Name:
- 2-{[(6-oxocyclohexa-2,4-dien-1-ylidene)methyl]amino}guanidine; undecan-2-one
- Test material form:
- liquid: viscous
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Fresh activated sludge was used as the inoculum. The activated sludge was obtained from a domestic wastewater treatment facility in New Jersey, USA. This treatment facility was selected because it deals predominantly with domestic sewage as specified in the guideline. There were no known contaminants in the fresh activated sludge believed to be present at levels high enough to interfere with this study. Fresh activated sludge was obtained on day -1 of the test. The activated sludge was homogenized in a blender for two minutes at medium speed. The homogenated sample was allowed to settle for approximately 30 minutes, after which the supernatant was decanted (avoiding carry-over of sludge solids). An aliquot of the supernatant was used to determine microbial activity. The microbial activity was determined using an Easicult®-TTC dip slide. The agar stick was removed from the culturing tube and the agar dipped into the supernatant aliquot. Excess supernatant was blotted off with a clean paper towel, and the agar stick was then placed back into the culture tube. The whole unit was placed into an environmental chamber for 24 hours at approximately 21 degrees C. The remaining decanted sludge supernatant was used for final preparation of the test medium on day -1.
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- ca. 34 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Biodegradation was based on oxygen consumption and the theoretical oxygen demand of the test substance as calculated using results of an elemental analysis of the test substance. The empirical formula and the theoretical oxygen demand (ThOD) of the test substance were calculated from elemental analysis data (assuming 100 gram test substance). Sodium benzoate ThOD was calculated using the empirical formula and was determined to be 1.66 mg O2/mg sodium benzoate. The ThOD calculation of the test and positive control substance was based on the OECD 301F guideline.The microbial count of the activated sludge was 10E4 CFU/mL. The sludge supernatant was added at a 1% loading volume to test medium. Test medium consisted of glass distilled water and mineral salts (phosphate buffer, ferric chloride, magnesium sulfate, calcium chloride). Test medium and activated sludge, which was aerated for 24 hours with carbon dioxide free air, was added to each respirometer flask.Test vessels were glass flasks placed in a water bath and electronically monitored for oxygen consumption. Triplicate test systems were run for the test substance; the positive control and the blanks.Test substance concentration was~50 mg/L. Test temperature was 21 – 23 deg C.All test vessels were stirred constantly for 28 days using magnetic stir bars.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
- Test performance:
- No deviations from the protocol were noted. By day 3, >60% biodegradation of positive control was observed, which meets the guideline requirement.
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 23.7
- St. dev.:
- 6.85
- Sampling time:
- 10 d
- Remarks on result:
- other: Based on ThOD
- Parameter:
- % degradation (O2 consumption)
- Value:
- 42.83
- St. dev.:
- 4.73
- Sampling time:
- 20 d
- Remarks on result:
- other: Based on ThOD
- Parameter:
- % degradation (O2 consumption)
- Value:
- 47.58
- St. dev.:
- 2.46
- Sampling time:
- 28 d
- Remarks on result:
- other: Based on ThOD
- Parameter:
- % degradation (O2 consumption)
- Value:
- 93.19
- St. dev.:
- 1.43
- Sampling time:
- 22 d
- Remarks on result:
- other: Based onThOD of dilithium glutarate
- Parameter:
- % degradation (O2 consumption)
- Value:
- 93.85
- St. dev.:
- 1.91
- Sampling time:
- 28 d
- Remarks on result:
- other: Based on ThOD
- Details on results:
- Test material was not readily biodegradable. By day 28, 47.6% degradation of the test material was observed.
BOD5 / COD results
- Results with reference substance:
- Sodium benzoate biodegraded 67% after 4 days (range 60.8 to 63.0), and 89% in 28 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance is not readily biodegradable in an OECD 301F Manometric Respirometry test.
- Executive summary:
The test substance is not readily biodegradable in an OECD 301F Manometric Respirometry test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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