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EC number: 244-034-6 | CAS number: 20780-49-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Remarks:
- experimental data of read across substances
- Justification for type of information:
- Data for the target chemical is summarized based on the structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- WoE report is based on two short term toxicity to aquatic invertebrates studies as-
2. and 3. - GLP compliance:
- not specified
- Analytical monitoring:
- no
- Remarks:
- 2. no and 3. yes
- Details on sampling:
- 2. no data available
3. - Concentrations: Test chemical conc. used for the study were 19.1, 34.3, 61.7, 111 and 200 mg/L (geometric ratio1.8), control. - Vehicle:
- no
- Remarks:
- 2. no and 3. not specified
- Details on test solutions:
- 2. The stock solution 100 mg/l was prepared by dissolving colourless liquid in reconstituted water. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with reconstituted test water.
3. no data available - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 2.
TEST ORGANISM
- Common name: Water flea
- Strain: Straus
- Source: Own breeding at University of Chemistry and Technology, Prague
- Age at study initiation (mean and range, SD): The animals used for the test shall be less than 24 h old and should not be first brood progeny
- Feeding during test: No feeding
3. no data available - Test type:
- other: 2. static and 3. semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- ± 1 hr
- Test temperature:
- 20±1°C
- pH:
- 2. without adjustment
sample at concentration 50 mg/l : pH = 7.9 changed to pH = 7.8 during the test
control: pH = 7.9 did not change during the test
3. no data available - Dissolved oxygen:
- 2. higher than 7.7 mg/L at the end of test both in the control and the sample
3. no data available - Nominal and measured concentrations:
- 2. Nominal test chemical conc. used for the were 6, 10, 17, 29 and 50 mg/l, respectively.
3. 19.1, 34.3, 61.7, 111 and 200 mg/L - Details on test conditions:
- 2.
TEST SYSTEM
- Test vessel: 50 ml glass vessel
- fill volume: 25 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural water (surface or ground water), reconstituted water or dechlorinated tap water are acceptable as culturing and dilution water if D. magna survives in it for the duration of the culturing, acclimation and testing without showing signs of stress. Waters in the range pH 6 to pH 9, with hardness between 140 mg/l and 275 mg/l (as CaCO3) are recommended.
As an example, the preparation of dilution water meeting the requirements is described below.
Dissolve known quantities of reagents in water. The dilution water prepared shall have a pH of 7.8 ± 0.5, a hardness of (225 ± 50) mg/l (expressed as CaCO3), a molar Ca + Mg ratio close to 4 + 1 and a dissolved oxygen concentration above 7 mg/l.
Prepare the solutions specified below:
- Calcium chloride solution: Dissolve 117.6 g of calcium chloride dihydrate (CaCl2.2H2O) in water (4.2) and make up to 1 l with water (4.2).
- Magnesium sulfate solution: Dissolve 49.3 g of magnesium sulfate heptahydrate (MgSO4.7H2O) in water (4.2) and make up to 1 l with water (4.2).
- Sodium bicarbonate solution: Dissolve 25.9 g of sodium bicarbonate (NaHCO3) in water (4.2) and make up to 1 l with water (4.2).
- Potassium chloride solution: Dissolve 2.3 g of potassium chloride (KCI) in water (4.2) and make up to 1 l with water (4.2).
Mixing
Mix 2.5 ml of each of the four solutions and make up to 1 l with water.
The dilution water shall be aerated until the dissolved oxygen concentration has reached saturation and the pH has stabilized. If necessary, adjust the pH to 7.8 ± 0.5 by adding sodium hydroxide (NaOH) solution or hydrochloric acid (HCI). The dilution water prepared in this way shall not be further aerated before use.
- Sodium hydroxide solution, e.g. [NaOH] : 1 mol/l.
- Hydrochloric acid, e.g. [HCl] : 1 mol/l.
Reference substance:
Dissolve 600 mg of potassium dichromate (K2Cr2O7) in water and make up to 1 l with water (4.2).
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment done
- Photoperiod: Darkness
CALCULATION:
EC50 was calculated using non linear regression by the software Prism 4.0
3.
TEST SYSTEM
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: test liquid volume ca. 250 mL per vessel
- No. of organisms per vessel: 20 per conc. (5 per vessel)
- No. of vessels per concentration (replicates): 4 replicates
OTHER TEST CONDITIONS
- Photoperiod: room light, 16 hr light – 8 hr dark
TEST CONCENTRATIONS
- Test concentrations: Test chemical conc. used for the study were 19.1, 34.3, 61.7, 111 and 200 mg/L (geometric ratio1.8), control. - Reference substance (positive control):
- yes
- Remarks:
- 2. Potassium dichromate (K2Cr2O7) and 3. no data available
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 18.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 2. 95 % C. I. 16.4 to 21.3 mg/l
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 24.6 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 3. 95% C. I. – 15.5 to 29.2 mg/l (Measurement concentration (time-weighted average value) was adopted for calculation of effect concentration, because some measurement concentrations exceeded ±20 % of setting).
- Results with reference substance (positive control):
- 2. - Results with reference substance valid
- EC50: 0.73 mg/L (24 hours)
3. no data available - Reported statistics and error estimates:
- 2. EC50 was calculated using non linear regression by the software Prism 4.0
3. no data available - Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value the test chemical on the mobility of the test organism Daphnia magna can be expected to be ranges from 18.7 to 24.6 mg/l, respectively. Thus, based on the EC50 value, test chemical can be considered as toxic to aq. invertebrate. Since the test chemical is readily biodegradable in water, test chemical can be considered as non-toxic to aq. invertebrate and thus can be considered to be not classified as per CLP classification criteria.
- Executive summary:
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic invertebrates.The studies are as mentioned below:
An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202 “Daphnia sp.,Acute Immobilization Test”. Own breeding stock at University of Chemistry and Technology, Prague ofDaphnia magnawas used as a test organism for the study.The stock solution 100 mg/l was prepared by dissolving colourless liquid in reconstituted water. Test solutions of required concentrations, i.e, 6, 10, 17, 29 and 50 mg/l were prepared by mixing the stock solution of the test sample with reconstituted test water. Study was performed using total 100 daphnids (5 organisms per vessel/replicates) in a static fresh water system. Daphnids were exposed to test chemical in 50 ml glass vessel in a volume of 25 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±1°C, pH 7.9 in dark conditions, respectively. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. On the basis of the effect of test chemical on the mobility of the test organism Daphnia magna, the 48 hr EC50 value was determined to be 18.7 mg/l with a 95% confidence interval value ranging from 16.4 to 21.3 mg/l, respectively.
In an another study, short term toxicity test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism for the study. Test chemical conc. used for the study were 19.1, 34.3, 61.7, 111 and 200 mg/L (geometric ratio1.8), control. Analytical monitoring involve the use of HS-GC. Study was performed using 20 per conc. (5 per vessel) Daphnia magna in a semi-static fresh water system. Total 100 fishes were exposed to test chemical in a closed vessel.The test vessels were placed in a room at a temperature of 20±1°C under a photoperiod of room light, 16:8 hr light: dark conditions, respectively.On the basis of mobility of the test organism Daphnia magna,the 48 hrs LC50 value was determined to be 24.6 mg/l (95% C. I. – 15.5 to 29.2 mg/l), respectively.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value the test chemical on the mobility of the test organism Daphnia magna can be expected to be ranges from 18.7 to 24.6 mg/l, respectively. Thus, based on the EC50 value, test chemical can be considered as toxic to aq. invertebrate. Since the test chemical is readily biodegradable in water, test chemical can be considered as non-toxic to aq. invertebrate and thus can be considered to be not classified as per CLP classification criteria.
Reference
Description of key information
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value the test chemical on the mobility of the test organism Daphnia magna can be expected to be ranges from 18.7 to 24.6 mg/l, respectively. Thus, based on the EC50 value, test chemical can be considered as toxic to aq. invertebrate. Since the test chemical is readily biodegradable in water, test chemical can be considered as non-toxic to aq. invertebrate and thus can be considered to be not classified as per CLP classification criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 18.7 mg/L
Additional information
Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxic effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:
An acute immobilisation test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD guideline No. 202 “Daphnia sp.,Acute Immobilization Test”. Own breeding stock at University of Chemistry and Technology, Prague of Daphnia magna was used as a test organism for the study. The stock solution 100 mg/l was prepared by dissolving colourless liquid in reconstituted water. Test solutions of required concentrations, i.e, 6, 10, 17, 29 and 50 mg/l were prepared by mixing the stock solution of the test sample with reconstituted test water. Study was performed using total 100 daphnids (5 organisms per vessel/replicates) in a static fresh water system. Daphnids were exposed to test chemical in 50 ml glass vessel in a volume of 25 ml of liquid solution containing both the chemical and media. The beakers were placed in a room at a temperature of 20±1°C, pH 7.9 in dark conditions, respectively. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. On the basis of the effect of test chemical on the mobility of the test organism Daphnia magna, the 48 hr EC50 value was determined to be 18.7 mg/l with a 95% confidence interval value ranging from 16.4 to 21.3 mg/l, respectively.
In an another study, short term toxicity test was conducted for 48 hrs for assessing the effect of test chemical on Daphnia magna. The test was performed in accordance to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Daphnia magna was used as a test organism for the study. Test chemical conc. used for the study were 19.1, 34.3, 61.7, 111 and 200 mg/L (geometric ratio1.8), control. Analytical monitoring involve the use of HS-GC. Study was performed using 20 per conc. (5 per vessel) Daphnia magna in a semi-static fresh water system. Total 100 fishes were exposed to test chemical in a closed vessel. The test vessels were placed in a room at a temperature of 20±1°C under a photoperiod of room light, 16:8 hr light: dark conditions, respectively. On the basis of mobility of the test organism Daphnia magna, the 48 hrs LC50 value was determined to be 24.6 mg/l (95% C. I. – 15.5 to 29.2 mg/l), respectively.
On the basis of the experimental studies of the structurally and functionally similar read across chemical and applying the weight of evidence approach, the EC50 value the test chemical on the mobility of the test organism Daphnia magna can be expected to be ranges from 18.7 to 24.6 mg/l, respectively. Thus, based on the EC50 value, test chemical can be considered as toxic to aq. invertebrate. Since the test chemical is readily biodegradable in water, test chemical can be considered as non-toxic to aq. invertebrate and thus can be considered to be not classified as per CLP classification criteria.
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