4'-Bromomethylbiphenyl-2-carbonitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 17 to 19, 1995

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure according to national standards but GLP uncertified

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: AFNOR French Standard T90 301 "Essais des eaux" (1983)

Deviations:

no

Principles of method if other than guideline:

In accordance with the AFNOR French Standard T90 301 "Essais des eaux : détermination de l'inhibition de la mobilité de Daphnia Magna", the aim of the study was to determine the EC50 48h of daphnia based on their mobility.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

425-280-5

EC Name:

-

Cas Number:

114772-54-2

Molecular formula:

Hill formula: C14 H10 Br N CAS formula: C14 H10 Br N

IUPAC Name:

4'-(bromomethyl)-[1,1'-biphenyl]-2-carbonitrile

Details on test material:

- Lot/batch No.: 5ARL004
- Physical state: non soluble powder

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnid
- Strain: Daphnia magna Straus
- Source: INERIS laboratories
- Age at study initiation (mean and range, SD): < 24h

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20°C +/- 2

pH:

8 +/- 0.2

Nominal and measured concentrations:

0.52, 0.73, 1.0, 1.4 & 2.0 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 10 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per vehicle control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Conductivity: 700 +/- 20 µS/cm
- Culture medium different from test medium: yes (water vs. DMSO)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.3 mg/L

Nominal / measured:

not specified

Conc. based on:

dissolved

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limits: 0.9-1.9 mg/l

Details on results:

- Mortality of control: None

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- EC50/LC50: 1.40 mg/l (1.29-1.54 mg/l)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

a) Oxygen concentration, when measured at the end of the test, is above 2 mg/l; b) the % of daphnia immoblised in the control is < 10; c) EC50 is found between the confidence limits recommended by the guideline: 0.9-1.5 mg/l

Conclusions:

The dissolved substance has a EC50 48 h = 1.3 mg/l.