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EC number: 947-719-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007-02-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
Sludge was taken from the activation basin of the ESN (Stadtentsorung Neustadt) sewage treatment plant, Im Altenschemel, 67435 NW-Laken-Speyerdorf
- Pretreatment: The sludge was washed with tap water three times, then centrifuged and re-suspended in tap water. 200 mL of the sludge were added to each test vessel.
- Initial biomass concentration: Dry matter in the test = 1518 mg suspended solids/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- not applicable
- Hardness:
- no data
- Test temperature:
- 20 +/- 2 deg C
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Conductivity:
- no data
- Nominal and measured concentrations:
- nominal: 1, 10, 100, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers
- Aeration: yes, approx 0.75 L/min
- No. of vessels per concentration (replicates): 2 for 1000 mg/L, 1 for 1-100 mg/L
- No. of vessels per control (replicates): 2
- No. of vessels per abiotic control (replicates):
- Sludge concentration (weight of dry solids per volume): 1518 mg/L
- Nutrients provided for bacteria: Synthetic sewage
- Biomass loading rate:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: Respiration rate
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
No
- Coefficient of variation of oxygen uptake rate in control replicates: Maximum of 8%, which is below the recommended limit of 15% - Results with reference substance (positive control):
- - Results with reference substance valid? yes, EC50 = 5.1 mg/L which is within the recommended range of 5-30 mg/L
- Reported statistics and error estimates:
- not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-h EC50 > 1000 mg/L
The 3-h NOEC = 1000 mg/L - Executive summary:
The inhibitory effect of the test item on the respiration rate of activated sludge microorganisms was determined according to OECD TG 209. The test item was tested using 5 concentrations ranging from 1 to 1000 mg/L. Duration of the test was 3 hours. Activated sludge taken from a domestic sewage treatment plant was used as inoculum. After washing, the dry matter was determined as 3.79 g suspended solids/L, giving a concentration of 1.52 g suspended solids/L in the test.
3,5 -dichlorophenol was used as the reference item. Five concentrations were tested; an EC50 of 5.1 was determined, which lies within the range of 5 -30 mg/L.
At 1000 mg/L no inhibition occurred. Therefore the pre-test can be taken as a limit test.
3h NOEC = 1000 mg/L
3h EC50 > 1000 mg/L
Reference
The test item showed very little inhibition in the experiment. In the highest treatment (1000 mg/L) no inhibition could be detected. In the the lower treatments, inhibition values stayed below 5%. Therefore, these values were not attributed to an inhibitory effect of the test item.
Description of key information
3h NOEC = 1000 mg/L
3h EC50 > 1000 mg/L
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The inhibitory effect of the test item on the respiration rate of activated sludge microorganisms was determined according to OECD TG 209. The test item was tested using 5 concentrations ranging from 1 to 1000 mg/L. Duration of the test was 3 hours. Activated sludge taken from a domestic sewage treatment plant was used as inoculum. After washing, the dry matter was determined as 3.79 g suspended solids/L, giving a concentration of 1.52 g suspended solids/L in the test.
3,5 -dichlorophenol was used as the reference item. Five concentrations were tested; an EC50 of 5.1 was determined, which lies within the range of 5 -30 mg/L.
At 1000 mg/L no inhibition occurred. Therefore the pre-test can be taken as a limit test.
3h NOEC = 1000 mg/L
3h EC50 > 1000 mg/L
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