Furan, 2-(ethoxymethyl)tetrahydro-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The work described was performed in compliance with UK GLP standards (Schedule 1, Good Laboratory Practice Regulations 1997 (SI 1997/654)). These Regulations are in accordance with GLP standards published as OECD Principles on Good Laboratory Practice (revised 1997, ENV/MC/CHEM(98)17); and are in accordance with, and implement, the requirements of Directives 87/18/EEC and 88/320/EEC.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Details on test solutions:

For the purpose of the definitive study the test material was prepared by a direct solution in water.
An amount of test material (200 mg) was dissolved in reconstituted water and the volume adjusted to 2 litres to give the 100mg/l test concentration.
The concentration and stability of the test material in the test solutions were verified by chemical analysis at 0 and 48 hours.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Daphnia Magna
- Source: Maintained in a laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquee (IRCHA), France

Total exposure duration:

48 h

Test temperature:

Maintained at 21°C throughout the study.

pH:

- 7.7 - 7.8
- No treatment related differences for pH were observed throughout the study.
- pH were recorded at the start and termination of the study.

Dissolved oxygen:

- 8.1 - 8.4
- No treatment related differences for oxygen concentraion were observed throughout the study.
- Dissolved oxygen concentrations were recorded at the start and termination of the study.

Nominal and measured concentrations:

Water temperature was recorded daily throughout the study.

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

>= 100 mg/L

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

RESULTS

Range-finding Study

Cumulative immobilisation data from the exposure of Daphnia magna to the test material during the range-finding study are given in Table 1.

Table 1: CUMULATIVE IMMOBILISATION DATA IN THE RANGE-FINDING STUDY

Nominal concentration (mg/l)	Cumulative immobilised Daphnia (initial population: 10 per replicate)
	24 hours	48 hours
Control	0	0
1.0	0	0
10	0	0
100	0	0

No immobilisation was observed at the test concentrations of 1.0, 10 and 100 mg/l.

Based on this information, a single test concentration of four replicates, of 100 mg.l was selected for the definitive study. This experimental design conforms to a "Limit test" to confirm that at the maximum test concentration given in the OECD/EEC guidelines no immobilisation or adverse reactons to exposure were observed.

Definitive Study

>Immobilisation data: Cumulative immobilisation data from the exposure of Daphnia magna to Ethyl tetrahydrofurfuryl ether during the definitive study are given in Table 2.

Table 2: CUMULATIVE IMMOBILSATION DATA IN THE DEFINTIVE STUDY

Nominal concentration (mg/l)	Cumulative immobilised Daphnia (initial population: 10 per replicate)
	24 hours			48 hours
	No. per replicate	Total	%	No. per replicate	Total	%
Control R1	0	0	0	0	0	0
Control R2	0	0	0	0	0	0
100 R1	0	0	0	0	0	0
100 R2	0	0	0	0	0	0
100 R3	0	0	0	0	0	0
100 R4	0	0	0	0	0	0

R₁ - R₄ = Replicates 1 -4

There was no immobilisation in 40 daphnids exposed to a test concentration of 100mg/l for a period of 48 hours. inspection of the immobilisation data gave the following results:

Time (h)	EC50 (mg/l)	95% Confidence limits (mg/l)
24	>100	-
48	>100	-

The No Observed Effect Concentration after 24 and 48 hours exposure was 100 mg/l. The No Observed Effect concentration is based upon a zero immobilisation at this concentration.

It was considered unnecessary and unrealistic to test at concentrations in excess of 100 mg/l.

>Physico-chemical measurements: Temperature was maintained at 21°C throughout the study, and no treatment related differences for oxygen concentration or pH were observed throughout the study. Results are given below.

PHYSICO-CHEMICAL MEASUREMENTS

Nominal concentration (mg/l)	0 hours			24 hours	48 hours
	pH	mg O2/l	T°C	T°C	pH	mg O2/l	T°C
Control R1	7.8	8.4	21.0	21.0	7.7	8.2	21.0
Control R2	7.8	8.4	21.0	21.0	7.8	8.1	21.0
100 R1	7.8	8.3	21.0	21.0	7.8	8.2	21.0
100 R2	7.8	8.4	21.0	21.0	7.8	8.2	21.0
100 R3	7.8	8.4	21.0	21.0	7.8	8.2	21.0
100 R4	7.8	8.4	21.0	21.0	7.8	8.2	21.0

R₁- R₄= Replicates 1 -4

>Verification of test concentrations: Analysis of the test preparations at 0 and 48 hours (refer to attached pdf report no. 905/028) showed the measured test concentrations to be near nominal and so it was considered justifiable to estimate the EC₅₀ values in terms of the nominal test concentrations only.

Conclusions:

The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been invesitgated and gave a 48-Hour EC50 of greater than 100 mg/l. Correspondingly the No Observed Effect Concentration was equal to 100 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

The acute toxicity of the test material to the freshwater invertebrate Daphnia magna has been invesitgated and gave a 48-Hour EC50 of greater than 100 mg/l.

Correspondingly the No Observed Effect Concentration was equal to 100 mg/l.