Isooctadecanoic acid, reaction products with tetraethylenepentamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 21, 1996- July 29, 1997

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP study conducted in compliance with the following OECD Method 202. Acceptable restriction was that analytical measurements on the test material were not conducted.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

no

Details on test solutions:

WAFs were prepared by combining the appropriate amount of test substance and dilution water in a mixing vessel equipped with a small magnetic stirrer (the vortex extended from the surface approximately 5% of the way to the bottom of the mixing vessel), stirring these mixtures at room temperature for approximately 24 hours, settling the mixtures for approximately four hours and siphoning the water phase containing the WAF.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnid
- Strain: Daphnia magna
- Source: Juvenile daphnids were produced from an in-house culture and identified using an approximate taxonomic key. The original parental culture was procured from Aquatic Biosystems, Inc., Fort Collins, Colorado on March 25, 1997
- Age at study initiation (mean and range, SD): Juvenile. Daphnids used for the test were less than 24 hours old.
At the conclusion of the test the control daphnids had an average wet weight of 0.21 mg
- Feeding during test: Daphnid cultures were supplied with the freshwater alga, Selenastrum capricornutum, and/or a yeast/trout chow mixture daily throughout the acclimation period. Daphnids were not fed during the test.
- Food type: Daphnid cultures were supplied with the freshwater alga, Selenastrum capricornutum, and/or a yeast/trout chow mixture
- Frequency: Daphnids were not fed during the test. Daphnid cultures were supplied with the freshwater alga, Selenastrum capricornutum, and/or a yeast/trout chow mixture daily throughout the acclimation period

ACCLIMATION
- Acclimation period: 14 day period
- Acclimation conditions (same as test or not): The culture temperature was 19 to 20.1 degrees Celsius and the dissolved oxygen concentration was always at least 7.4 mg/L
- Type and amount of food: Daphnid cultures were supplied with the freshwater alga, Selenastrum capricornutum, and/or a yeast/trout chow mixture daily throughout the acclimation period
- Feeding frequency: Daphnid cultures were supplied with the freshwater alga, Selenastrum capricornutum, and/or a yeast/trout chow mixture daily throughout the acclimation period
- Health during acclimation (any mortality observed): During acclimation daphnids were not treated for disease and they were free of apparent sickness, injuries and abnormalities at the beginning of the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Water was adjusted to a hardness of 160 to 180 mg/L as CaCO3. Water used for the definitive test had a hardness of 172 mg/L

Test temperature:

20 +/- 1 degree Celsius

pH:

pH of <8

Dissolved oxygen:

Dissolved oxygen was always at least 7.4 mg/L

Nominal and measured concentrations:

A range finding test was conducted with dilution water control and the WAF of four concentrations of test material: 1, 10, 100 and 1000 mg/L. The final definitive test was conducted with a dilution water control and the WAF of five concentrations of test material: 130, 220, 360, 600 and 1000 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 ml beakers
- Type: loosely covered
-Aeration: Not required
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Wated used for acclimation of test organisms and for all toxicity testing was deionized tap water collected at Marblehead, Massachusetts.
- Total organic carbon: not detected at or above the limit of quantitation (1.0 mg/L)
- Metals: Calcium = 26.3 mg/L, Magnesium = 23.6 mg/L, Potassium = 3.92 mg/L, Sodium = 48.1 mg/L
- Pesticides: Not detected at the limit of quantitation for any
- Chlorine: < 0.01 mg/L
- Alkalinity: 70 mg/L as CaCO3
- Intervals of water quality measurement: Dissolved oxygen concentration, pH, conductivity and temperature were measured in each test vessel initially and at 24 hour intervals. The measured concentrations of TOC were determined in samples of test media collected at the beginning and end of the 48 hours of the toxicity test.

OTHER TEST CONDITIONS
- Adjustment of pH: <8
- Photoperiod:16 hour light and 8 hour dark photoperiod
- Light intensity: 2 uEin/m2 sec

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The number of surviving organisms and the presence of sublethal effects was determined visually and recorded intially and after 2, 24 and 48 hours.


TEST CONCENTRATIONS

- Range finding study
- Test concentrations: 1, 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: There was 100% survival in the control and at all 4 tested concentrations in the range finder. Becuase of this 5 WAF concentrations for the definitive study were used: 130, 220, 360, 600 and 1000 mg/L.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Details on results:

No insoluble material was noted during the test. One hundred percent survival with no sublethal effects occurred in the control. The mean dissolved oxygen concentration during the definitive test was 8.3 mg/l (range = 8.0 to 8.8 mg/L), the mean conductivity was 580 umhos/cm (range = 570 to 580 mg/L), the mean temperature was 20 degrees Celsius (range = 19.6 to 20.6 degrees Celsius), and the pH ranged from 7.6 to 7.9.

The 24 and 48 hour EC50s are greater than 1,000 mg/L of test material (the highest tested concentration), and the 48-hour NOEC is 1,000 mg/L.

Reported statistics and error estimates:

Results of the toxicity tests could not be interpreted by standard statistical techniques because at least 90% survival occurred in all tested concentrations. The no observed effect concentration is the highest concentration of test substance that allowed at least 90% survival of exposed organisms and did not cause sublethal effects.

Validity criteria fulfilled:

yes

Conclusions:

Exposure of daphnids, Daphnia magna, to the WAF of test material resulted in a 48 -hour median effective concentration (EC50) greater than 1,000 mg/L (the highest tested concentration), based on nominal concentrations. The 48 -hour no observed effect concentration (NOEC) is 1,000 mg/L of test material, based on nominal concentrations.

Executive summary:

The acute toxicity of the water accommodated fraction (WAF) of five mixtures of the test material and water to the daphnid, Daphnia magna, was investigated during a study conducted at T.R. Wilbury Laboratories, Inc. The test, which was designated to determine the toxicity of the test substance, was performed from May 29 to 31, 1997.

The test was performed at 20 +/- 1 degree Celsius under static conditions with a control and five nominal concentrations of test substance (130, 220, 360, 600 and 1000 mg/L). The dilution water was carbon-filtered deionized water collected at Marblehead, Massachusetts and adjusted to a hardness of 172 mg/L as CaCO3 and a pH of <8.0. Water quality was within acceptable limits throughout the test and no insoluble material was noted during the test. Juvenile daphnids (less than 24 hours old) produced by an in-house culture that was maintained at test conditions were used to initiate the test.

Exposure of daphnids, Daphnia magna, to the WAF of test material resulted in a 48 -hour median effective concentration (EC50) greater than 1,000 mg/L (the highest tested concentration), based on nominal concentrations. The 48 -hour no observed effect concentration (NOEC) is 1,000 mg/L of test material, based on nominal concentrations.

Description of key information

Based on results from an acceptable OECD 202 test, EC 701-204-9 has an LC50>1000 mg/L and would not be classified as harmful to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

LC50

Effect concentration:

1 000 mg/L

Additional information

Two studies were available to determine the acute toxicity to aquatic invertebrates.

The Ward et al., 1997 study was selected as the key study because it was performed in line with GLP and accepted standardised guidelines. It was assigned a Klimisch score of 2, reliable with restriction. Acceptable restriction was that analytical measurements on the test material were not conducted. The acute toxicity of the test material at five concentrations of test material, 130, 220, 360, 600 and 1000 mg/L (WAF) were evaluated in Daphnia magna. Exposure of daphnids to the WAF of test material resulted in a 48 -hour median effective concentration (EC₅₀) greater than 1,000 mg/L (the highest tested concentration), based on nominal concentrations. The 48 -hour no observed effect concentration (NOEC) is 1,000 mg/L of test material, based on nominal concentrations.

The Ward et al., 1994 study was disregarded due to the appearance of small suspended particles in non-control test vessels

throughout the test. Therefore, this study was assigned a Klimisch Code rating of 3, not reliable.