1,4-bis[(4-butylphenyl)amino]-5,8-dihydroxyanthraquinone

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from review article or handbook

Data source

Reference

Reference Type:

review article or handbook

Title:

4,4'-methylenebis (2,6-di-tert-butylphenol) [MAK Value Documentation in German language, 2014].

Author:

The MAK Collection for Occupational Health and Safety.

Year:

2014

Bibliographic source:

4,4'-methylenebis (2,6-di-tert-butylphenol) [MAK Value Documentation in German language, 2014]. The MAK Collection for Occupational Health and Safety. 1-24. 2014.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Skin irritation study of 4,4'-Methylenebis(2,6-di-tert-butylphenol)(CAS no: 118-82-1) was conducted on intact and abraded skin of six New Zealand rabbits under semi-occlusive condition.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,2',6,6'-Tetra-tert-butyl-4,4'-methylenediphenol

Cas Number:

118-82-1

Molecular formula:

C29H44O2

IUPAC Name:

2,2',6,6'-Tetra-tert-butyl-4,4'-methylenediphenol

Test material form:

solid

Details on test material:

- Name of test material (as cited in study report): 4,4'-Methylenebis(2,6-di-tert-butylphenol)
- Molecular formula: C29H44O2
- Molecular weight: 424.665 g/mol
- Substance type: organic
- Physical state: solid
- InChI: 1S/C29H44O2/c1-26(2,3)20-14-18(15-21(24(20)30)27(4,5)6)13-19-16-22(28(7,8)9)25(31)23(17-19)29(10,11)12/h14-17,30-31H,13H2,1-12H3
-Smiles: C(c1c(c(C(C)(C)C)cc(c1)Cc1cc(C(C)(C)C)c(O)c(c1)C(C)(C)C)O)(C)(C)C

Specific details on test material used for the study:

- Name of test material (as cited in study report): 4,4'-Methylenebis(2,6-di-tert-butylphenol)
- Molecular formula: C29H44O2
- Molecular weight: 424.665 g/mol
- Substance type: organic
- Physical state: solid
- InChI: 1S/C29H44O2/c1-26(2,3)20-14-18(15-21(24(20)30)27(4,5)6)13-19-16-22(28(7,8)9)25(31)23(17-19)29(10,11)12/h14-17,30-31H,13H2,1-12H3
-Smiles: C(c1c(c(C(C)(C)C)cc(c1)Cc1cc(C(C)(C)C)c(O)c(c1)C(C)(C)C)O)(C)(C)C

Test system

Type of coverage:

semiocclusive

Preparation of test site:

other: Intact and abraded skin

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

500mg

Duration of treatment / exposure:

24 hours

Observation period:

24 and 72 hours

Number of animals:

6

Details on study design:

SCORING SYSTEM: Draize method

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24/48/72 h

Score:

0.05

Max. score:

4

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No known signs of skin irritation were observed.

Applicant's summary and conclusion

Interpretation of results:

other: Not irritating

Conclusions:

The test material 4,4'-Methylenebis(2,6-di-tert-butylphenol)(CAS no: 118-82-1) was considered to be not irritating to the skin of New Zealand rabbits.

Executive summary:

Skin irritation study of 4,4'-Methylenebis(2,6-di-tert-butylphenol)(CAS no: 118-82-1) was conducted on intact and abraded skin of six New Zealand rabbits under semi-occlusive condition.

 

About 500 mg of the4,4'-Methylenebis(2,6-di-tert-butylphenol)was applied for 24 hours under semiocclusive conditions. After 24 hours, the application site was scored at 24 or 72 hours according to Draize method.

 

No signs of irritation were observed and the observed irritation score was 0.5. Hence the chemical 4,4'-Methylenebis(2,6-di-tert-butylphenol)(CAS no: 118-82-1)was considered to be not irritating to the skin of New Zealand rabbits.