Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline followed. Study well conducted.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2007
Reference Type:
study report
Title:
Unnamed
Year:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: USEPA(2002) Methods for measuring the acute toxicity of effluents and receiving waters to freshwater organisms. Cincinnati, Ohio, U.S. Environmental Protection Agency
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Lead dinitrate
EC Number:
233-245-9
EC Name:
Lead dinitrate
Cas Number:
10099-74-8
Details on test material:
- Name of test material (as cited in study report): Pb

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
- Test organisms originate from Aquatic Biosystems and maintained in dechlorinated tapwater at 26°C
- For all tests, a mass culture was started and maintained for 3 days. All mass cultures were fed with a combination of YCT and algae
- Test organisms were < 24 h old before testing

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
16.4 mg/L
Test temperature:
25°C
pH:
5.71+-0.05
Nominal and measured concentrations:
Nominal conc. (µg/L Pb):0, 5, 10, 20, 40, 80
Measured conc. (µg/L Pb): 0, 3, 9, 17, 36, 76
Details on test conditions:

- 5 individuals/20 mL polyethylene cups
- 4 replicates were used for testing

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
73.56 µg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
dissolved
Basis for effect:
mortality
Remarks on result:
other: 95%CI: (µg/L Pb2+): 45.1 - 119.1
Reported statistics and error estimates:
All acute tests were assessed for LC50 values using Maximum Likelihood Probit or Trimmed Spearman Karber method

Any other information on results incl. tables

Water quality parameters in micromol:

Na+: 78; Ca2 +: 106; Mg2 +: 58; K+: 55; Cl-: 408; SO42 -: 21; DOC: 50

Applicant's summary and conclusion