3,4,5,6-tetrachloro-N-[2-(4,5,6,7-tetrachloro-2,3-dihydro-1,3-dioxo-1H-inden-2-yl)-8-quinolyl]phthalimide

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Principles of method if other than guideline:

5 mice per sex per dose were administerd a single dose of the test substance via intraperitoneal injection. Clinical signs and body weight were monitored and necropsy was carried out at termination (or after death, if it occured) on all animals. The animals were sacrificed on day 7 after application.

GLP compliance:

no

Remarks:

performed prior to the implementation of GLP

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,4,5,6-tetrachloro-N-[2-(4,5,6,7-tetrachloro-2,3-dihydro-1,3-dioxo-1H-inden-2-yl)-8-quinolyl]phthalimide

EC Number:

250-063-5

EC Name:

3,4,5,6-tetrachloro-N-[2-(4,5,6,7-tetrachloro-2,3-dihydro-1,3-dioxo-1H-inden-2-yl)-8-quinolyl]phthalimide

Cas Number:

30125-47-4

Molecular formula:

C26H6Cl8N2O4

IUPAC Name:

4,5,6,7-tetrachloro-2-[2-(4,5,6,7-tetrachloro-1,3-dioxo-2,3-dihydro-1H-inden-2-yl)quinolin-8-yl]-1H-isoindole-1,3(2H)-dione

Test material form:

solid: nanoform

Details on test material:

- Physical state/ appearance: solid / yellow
- Shape of particles: spherical
- Aspect ratio: 1.5
- Particle size distribution: 62.7 nm
- Crystal structure: crystalline
- Surface area of particles: 29.6 m^2/g
- Surface treatment: no

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Ivanovas
- Weight at study initiation (mean): 27 g (males), 23 g (females)

ENVIRONMENTAL CONDITIONS: not reported

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Remarks:

(0.5% aqueous solution)

Details on exposure:

35% solution of test substance in 0.5% CMC-solution

Doses:

6810 and 10000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: day 1 (several times), 5 and 8
- Necropsy of survivors performed: yes

Statistics:

not performed

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 10 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 1 animal died within the 7 day observation period.

Mortality:

1 animal died within 48 h after application of the test substance (10000 mg/kg bw).

Clinical signs:

Dyspnoea, apathy, tremor

Body weight:

- Mean weight (6810 mg/kg bw): day 1: 26 g (males), 20 g (females); day 5: 27 g (males), 24 g (females); day 8: 28 g (males), 23 g (females)
- Mean weight (10000 mg/kg bw): day 1: 27 g (males), 23 g (females); day 5: 27 g (males), 24 g (females); day 8: 30 g (males), 26 g (females)

Gross pathology:

10000 mg/kg bw intra-abdominal substance residues (10/10 animals); slight adhesions and spleen enlargement (9/10 animals)

6810 mg/kg bw intra-abdominal substance residues; slight adhesions and megaly of the spleen