Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 245-910-0 | CAS number: 23847-08-7
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
Activated sludge, acute toxicity test for microorganisms, EC 440/2008 Method C.11 (OECD 209), EC50 (3h) > 1000 mg/L
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The acute toxicity to microorganisms of the test substance was investigated according to EC 440/2008 Method C.11, which is equivalent to OECD 209 (Richter, 2011). The study was conducted under GLP. The inoculum was taken from the aeration tank of a domestic sewage treatment plant (Municipal STP Cologne - Stammheim) and was daily fed with synthetic medium. The substance was tested in the concentrations of 10, 100 and 1000 mg/L under flow-through conditions in freshwater. The temperature was held constant at 20 +/- 2°C at the pH range of 6 - 8. The dissolved test medium was mixed with the activated sludge, synthetic medium and deionised water. 3,5 -Dichlorophenol was used as reference substance and prepared in the same way. Two control vessels were prepared as well as vessels to determine the physico-chemical oxygen consumption. For this purpose no activated sludge is added to the mixture. After 3 h incubation time the respiration was inhibited for 32.2 % at a test item concentration of 1000 mg/L. The EC50 value (> 1000 mg/L) is calculated from respiration rates at different test item concentrations using the statistics program ToxRatPro Version 2.10 (released 2010 -02 -20). All validity criteria of the test method were met: the respiratory rates of the 2 controls differ less than 15 % from each other and the EC50 of the reference compound is in the range of 5 -30 mg/L (result: 10 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
