Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 442-070-9 | CAS number: 329039-38-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
Methyldiacetoxyisopropoxy silane was tested in four different GLP-Guideline studies for genetic toxicity.
Methyldiacetoxyisopropoxy silane was tested in an Ames test using Salmonella typhimurium strains TA 1535, TA 1537, TA 98, and TA 100 and the Escherichia coli strain WP2 uvrA . The substance was tested up to 5000 µg/plate with and without metabolic activation. No toxic effects occurred in all test groups, with and without S9 mix. Under the experimental conditions the test item did not induce mutations by base pair changes or frameshifts in the genome of the strains used (Wagner, 2001).
The test item methyldiacetoxyisopropoxy silane was assessed for its potential to induce structural chromosome aberrations in ovary cells of the Chinese hamster in vitro in two independent experiments. No toxic effects were observed after treatment with up to 5000 µg/mL of the test item. No statistically significant or biologically relevant increase in the number of cells carrying structural chromosomal aberrations was observed after treatment with the test item. No increase in the frequencies of polyploid metaphases was found after treatment with methyldiacetoxyisopropoxy silane compared to the frequencies of the controls (Gudi, 2001).
The test item methyldiacetoxyisopropoxy silane was assessed in a GLP study according to OECD 476 for its potential to induce mutations at the mouse lymphoma thymidine kinase locus using the cell line L5178Y.
The assay was performed in duplicates. The experiment was performed with and without liver microsomal activation and a treatment period of 4 h. The selection of the maximum concentrations of 5000 µg/mL without S9-mix and 4000 µg/mL with S9-mix were based on data from the pre-experiment. The test item was investigated at the following concentrations:
+S9: 1500, 2000, 2500, 3000 and 4000 µg/mL
-S9: 2000, 2500, 3000, 4000 and 5000 µg/mL
Relevant toxic effects indicated by a relative total growth of less than 50 % of relative total growth were observed in at 4000 µg/mL in the presence of metabolic activation. No biologically relevant increase of mutants was found after treatment with the test item (with and without metabolic activation). No dose-response relationship was observed. Appropriate reference mutagens were used as positive controls and showed a distinct increase in induced mutant colonies, indicating that the tests were sensitive and valid. In conclusion it can be stated that during the mutagenicity test described and under the experimental conditions reported the test item did not induce mutations in the mouse lymphoma thymidine kinase locus assay using the cell line L5178Y in the absence and presence of metabolic activation (San, 2001).
To investigate the potential of methyldiacetoxyisopropoxy silane to induce micronuclei in polychromatic erythrocytes (PCE) in the bone marrow of the mouse, a GLP study according to OECD 474 was performed.
Methyldiacetoxyisopropoxy silanewas prepared in either distilled water (first experiment) or corn oil (second experiment). The test substance was administered intraperitoneal at a volume of 20 mL/kg bw by a single injection.
Five animals per test group were evaluated for the occurrence of micronuclei. At least 2000 polychromatic erythrocytes (PCEs) per animal were scored for micronuclei.
Based on preliminary tests the following dose levels of the test item were investigated:
1. Experiment: 50, 100 and 200 mg/kg bw
2. Experiment: 87.5, 175 and 300 mg/kg bw.
In comparison to the corresponding vehicle controls there was no biologically relevant or statistically significant enhancement in the frequency of the detected micronuclei at any preparation interval after administration of the test item with any dose level used.
Thus, methyldiacetoxyisopropoxy silane did not induce the micronucleus frequency under the given test conditions (Gudi, 2001).
Short description of key information:
Genetic toxicity in-vitro in-vivo: negative
Genetic toxicity in-vivo: negative
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
According to DSD (67/548/EEC) and CLP (1272/2008/EC) methyldiacetoxyisopropoxy silane does not meet the criteria to be classified regarding this endpoint.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.