2-Naphthalenesulfonic acid, 6-amino-5-[[4-[[4-chloro-6-[ethyl[3-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulfophenyl]azo]-4-hydroxy-, sodiumsalt

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Principles of method if other than guideline:

Test procedure:
Species: Mouse, CBA strain, inbred, SPF-Quality.

Recognized by the international guidelines as the
recommended test system (e.g. OECD, EC, EPA).

Source: Charles River France, L'Arbresle Cedex, France

Number of animals: 20 females (nulliparous and non-
pregnant), five females per group.



In the main study, three groups of five experimental animals
were treated with test substance concentrations of 5%, 25%
or 50% on three consecutive days, by open application on the
ears. Five vehicle control animals were similarly treated,
but with vehicle alone (Dimethyl sulphoxide).

Three days after the last exposure, all animals were
injected with 3H-methyl thymidine and after five hours the
draining (auricular) lymph nodes were excised.

After precipitating the DNA of the lymph node cells,
radioactivity measurements were performed. The activity was
expressed as the number of Disintegrations Per Minute (DPM)
and a stimulation index was subsequently calculated for each
group.

Test material

Test material information

Constituent 1

Results and discussion

Details on results:

The SI values calculated for the substance concentrations 5,
25 and 50% were 9.4, 8.9 and 12.9 respectively.


These results show that the test substance elicits an SI >=
3. The EC3 value was established to be between 0 and 5%.