Ethanone, 1-(4-methoxyphenyl)-2-[(3-methoxyphenyl)thio]-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jun 2006 to Feb 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

469-300-0

EC Name:

-

Cas Number:

63675-73-0

Molecular formula:

Hill Empirical Formula: C16H16O3S CAS Empirical Formula: C16H16O3S

IUPAC Name:

1-(4-methoxyphenyl)-2-[(3-methoxyphenyl)sulfanyl]ethan-1-one

Test material form:

solid: particulate/powder

Details on test material:

Lot no: 151105Purity - 101.39%

Sampling and analysis

Analytical monitoring:

yes

Remarks:

HPLC

Details on sampling:

Concentrations of Beta Ketosulfide in fish water were determined by high performance liquid chromatography (HPLC) with UV detection at 280nm. Samples (20ml) of test media from each of the Beta Ketosulfide treatments and from the control treatments were taken for analysis at 0 and 72 hours from freshly perpared test media and at 24 and 96 hours from 24 hour old test media.

Test solutions

Vehicle:

yes

Remarks:

Water

Details on test solutions:

Dilution water used for conducting the definitive test was dechlorinated mains water which was passed through particulate and activated charcoal filters and treated with Ozone to remove residual biologically active content.The definitive / limit test was conducted as a semi-static test with test media prepared and renewed daily, at the nominal Beta Ketosulfide concentrations of 2 and 100mg/L These concentrations were based on the results of a range finding test and the practical limit of water solubility.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Healthy Oncorhynchus mykiss (rainbow trout) were supplied by a recognised supplier, whose details are maintained in the study raw data. Fish were acclimatised to the test conditions for longer than 12 days prior to testing. Fish were randomly selected from one of the holding tanks and allocated to the test vessels at the start of each phase of testing. At the end of the test, the fork length and mean wet weight, of ten fish selected at random from the remaining fish in the definitive test, was determined.

Study design

Test type:

semi-static

Water media type:

other: dechlorinate mains water

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

At 96 hours, all fish had died at 100mg/l. In the 2mg/L treatment, all fish were exhibiting severe toxic symptoms including darkened pigmentation, immobility and hyperventilation

Test conditions

Hardness:

see table below

Test temperature:

see table below

pH:

see table below

Dissolved oxygen:

see table below

Salinity:

see table below

Reference substance (positive control):

yes

Remarks:

positive controls

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.831 mg/L

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 0.831 mg/L

Details on results:

Beta Ketosulfide was not lethal to Oncorhynchus mykiss, at the practical limit of water solubility achieved during this test. The 24, 48, 72 and 96 hour acute toxicity LC50 values were greater than a nominal concentration of 2mg/L and greater than the mean measured soluble concentration of 0.831mg/l.A no observed effect concentration (NOEC) was considered to be less than a nominal concentration of 2mg/L and less than the mean measured soluble concentration of 0.831 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Beta Ketosulfide was not lethal to Oncorhynchus mykiss, at the practical limit of water solubility a chieved during this test. The 24, 48, 72 and 96 hour acute toxicity LC50 values were greater than a nominal concentration of 2mg/L and greater than the mean measured soluble concentration of 0.831mg/l.A no observed effect concentration (NOEC) was considered to be less than a nominal concentration of 2mg/L and less than the mean measured soluble concentration of 0.831 mg/l.

Executive summary:

The study was conducted in accordance with the requirements of OECD Chemicals Testing Guideline No. 203 Fish, Acute Toxicity Test (adopted 17 July 1992) and Annex V to EC directive 92/69/EEC Methods for the Determination of Ecotoxicity Part C: C.1. Acute Toxicity for Fish. The overall mean measured concentrations of Beta Ketosulfide were 0.831 and 5.38 mg/L at the nominal 2 and 100mg/l concentrations respectively. At the practical limit of water solubility achieved during this test. The 24, 48, 72 and 96 hour acute toxicity LC50 values were greater than a nominal concentration of 2mg/L and greater than the mean measured soluble concentration of 0.831mg/l.