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Diss Factsheets
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EC number: 701-085-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The initial cell density of ~ 70000/mL was distinctly above the recommended one of 10000/mL.
- GLP compliance:
- no
Test material
- Reference substance name:
- Silicic acid, calcium salt
- EC Number:
- 215-710-8
- EC Name:
- Silicic acid, calcium salt
- Cas Number:
- 1344-95-2
- IUPAC Name:
- Silicic acid, Calcium salt
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Name of test material (as cited in study report) = Shieldex AC 3
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Aqueous extracts of loadings (water-accommodated fraction, WAF)
after 24-h shaking of the suspensions in deionised water at approx. 130 revolutions/min;.
fitration through folded paperfilter;
addition of necessary salts from stock solutions before test
- Differential loading: yes
- Controls: yes, without respective filtrates, but with algae
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Filtrates (WAFs) were clear.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- Strain: Scenedesmus subspicatus CHODAT
- Source (laboratory, culture collection): Institute of Plant Physiology, University Göttingen
- Age of inoculum (at test initiation): 3 d, actively growing cell culture
- Method of culture: preculture 3 days prepared before test from a stock culture
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- no data
- Test temperature:
- 25 ± 1 °C
- pH:
- control: 8.29 (initial); 9.97 (final)
tests: 8.35 --> 9.43 (100 mg/L)
8.66 --> 8,44 (200 mg/L)
9.04 --> 8.39 (400 mg/L)
9.16 --> 8.49 (800 mg/L)
9.19 --> 8.73 (1000 mg/L)
- Dissolved oxygen:
- not applicable
- Salinity:
- no data
- Nominal and measured concentrations:
- 100, 200, 400, 800, and 1000 mg/L (nominal, WAFs of loadings)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: no data
- Initial cells density: ~ 72000/mL
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 7
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: continuous
- Light intensity and quality: > 120 µE/m2s; ~ 8000 Lux (400 - 700 nm)
EFFECT PARAMETERS MEASURED
- Determination of cell concentrations: spectrophotometer at 578 nm (cell density, extinction)
- Chlorophyll measurement: no
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 100; 1000; and 10000 mg/L (loadings) - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 41 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 33 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 410 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no data
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no, WAFs were used - Reported statistics and error estimates:
- No standard deviations of mean cell concentrations reported. 95%-Confidence limits graphically given in the growth curve plot.
No statistical evaluation available.
Any other information on results incl. tables
Average cell densities and inhibition values over time (from Report, Sections 7.1 and 7.2)
Concentration [mg/L] |
Cell density*) after time of incubation |
% inhibition after 72 h |
|||
24 h |
48 h |
72 h |
Biomass |
Growth rate |
|
Control (0) |
2.92 x105 |
1.14 x106 |
3.32 x106 |
0 |
0 |
100 |
2.38 x105 |
7.72 x105 |
1.98 x106 |
37.4 |
14.5 |
200 |
4.37 x105 |
8.55 x105 |
2.00 x106 |
27.4 |
30.2 |
400 |
3.92 x105 |
6.70 x105 |
1.48 x106 |
44.3 |
34.6 |
800 |
2.85 x105 |
4.97 x105 |
9.37 x105 |
63.2 |
36.9 |
1000 |
2.64 x105 |
4.25 x105 |
8.38 x105 |
68.1 |
39.8 |
*) Initial cell density = ~ 7.2 x104 cells/mL
Results of pre-testing not provided.
The EC10 value was extrapolated, but procedure not explicitely documented. However, the value is plausible.
The inhibitory effect showed a relatively weak dose-response relationship, with no unequivocal dose response
discernible from 100 to 400 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Remarks:
- Cell proliferation valid (~50-fold increase of initial over 72 h); statistical criteria for the validation parameters appear to be met.
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