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Diss Factsheets
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EC number: 941-122-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of 2,6-dimethylpyridinium chloride and 3-methylpyridinium chloride and 4-methylpyridinium chloride
- EC Number:
- 941-122-2
- Molecular formula:
- not yet assigned
- IUPAC Name:
- Reaction mass of 2,6-dimethylpyridinium chloride and 3-methylpyridinium chloride and 4-methylpyridinium chloride
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: LC-MS/MS
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The stock solution (100 mg/L of the test item were weighed out) was freshly prepared in a measuring flask with dilution water before the start of the exposure (at 0 h).
- Controls: Dilution water without test item tested under the same conditions as the test groups. 4 replicates, each with 5 daphnids.
- Evidence of undissolved material: The stock solution was prepared in a measuring flask and mixed thoroughly by inverting the measuring flask several times until the test item was completely dissolved.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Method of breeding: In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 2°C, in an incubator, 16 h illumination, light intensity of max. 20 µExm-2 x s-1
- Feeding during test
- Food type: mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus
- Amount: algae cell density of > 10^6 cells/mL
- Frequency: 5 times per week ad libitum
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- NaHCO3 64.8 mg/L
- Test temperature:
- 18 - 19 °C
- pH:
- test start: 6.2-7.61
test end: 6.48-6.98 - Dissolved oxygen:
- test start: 8.8-9.08 mg/L
test end: 7.82-8.62 mg/L - Nominal and measured concentrations:
- nominal: 100 mg/L
The measured concentration of the test item at the start of the exposure (0 h) was 100 % of the nominal value. At the end of the exposure (48 h), the measured concentration of the test item was 106 % of the nominal value. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
-Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with laboratory film (PARAFILM®)
- Test volume: 20 mL in each test replicate
- Aeration: no
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: double distilled water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 h light/dark cycle
- Light intensity: Diffuse light, light intensity of max. 20 µE x m^-2 x s^-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobilisation was monitored one and two days after exposure - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: all daphnids were mobile in the control during the 2 days of exposure.
- Any observations that might cause a difference between measured and nominal values: the test substance was soluble in medium, the test concentrations were present as a clear solution. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50/LC50: EC50 (24 h): 2.01 mg/L (CI 1.71 - 2.26 mg/L) with 95 % confidence limits (CI)
Any other information on results incl. tables
Table3: Immobilisation Rates after 24 and 48 h ofExposure in the Definitive Test (n = 20, divided into 4 replicates with 5 daphnids each)
Pyridinderivate 1_HCl Nominal test item concentration [mg/L] |
IMMOBILISATION [%] | |||||||||
24 h | 48 h | |||||||||
Replicates | Replicates | |||||||||
1 | 2 | 3 | 4 | MV | 1 | 2 | 3 | 4 | MV | |
100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
MV = mean value
The validation criteria were fulfilled as there were no immobilised daphnids in the control group and oxygen saturation, temperature, pH value as well as measured values for the test medium were in the required ranges.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the nominal concentration of 100 mg/L of the test item Pyridinderivate 1_HCl no effects were observed on Daphnia magna.
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