Phenolates from Hydrocarbon Resin production

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Data source

Reference

Reference Type:

publication

Title:

A four-day survival and reproduction test for Ceriodaphnia dubia

Author:

Oris, J.T.; Winner, R.W.; Moore, M.V.

Year:

1991

Bibliographic source:

Environ. Toxicol. Chem., 10, 217-224

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Principles of method if other than guideline:

Mortality test after 48h

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Phenol

EC Number:

203-632-7

EC Name:

Phenol

Cas Number:

108-95-2

Molecular formula:

C6H6O

IUPAC Name:

phenol

Specific details on test material used for the study:

Phenol obtained at a concentration of 80 % in water

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Method: Dilution from stock solution of phenol in test water.

Test organisms

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

. Common name: Ceriodaphnia
- Source: no information
- Age at study initiation (mean and range, SD): < 12 hours old neonates
- Method of breeding: at 25 +/- 1 °C on a 16:8 h light:dark photoperiod with 28 lux, cool, white fluorescence lamps.
Animals were held individually in 15 ml of moderately hard reconstituted water.

- Feeding during test: no feeding during the 48 h test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

hardness 57.07 ± 4.14 mg CaCO3/L
alkalinity 81.0 ± 4.22 mg CaCO3/L

Test temperature:

25 ± 1 °C

pH:

8.2

Dissolved oxygen:

no data

Salinity:

reconstituted water according to EPA 600/4-89/001A, 2nd ed.

Nominal and measured concentrations:

four concentrations, 6, 4, 2, and 1 mg phenol/L (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessel: test cups, not further specified
- Type (delete if not applicable): no information
- Material, size, headspace, fill volume: 30 mL
- Aeration: no
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: (reconstituted water with the addition of 2 µg Se/L as sodium selenite
- Alkalinity: 81 mg/L as CaCO3
- Ca/mg ratio: no data
- Conductivity: no data
- Culture medium different from test medium: no
- Intervals of water quality measurement: no information


OTHER TEST CONDITIONS
assumed to be comparable to breeding conditions
- Adjustment of pH: no
- Photoperiod: 16:8 h light:dark
- Light intensity: 28 lux


EFFECT PARAMETERS MEASURED: Mortality was recorded daily.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5 and 2

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

3.1 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality of control less than 10%.