Ethanone, 1-(1-chlorocyclopropyl)-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

06 Nov - 08 Nov 2002

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

TOC determination

Details on sampling:

- Concentrations: 6.3, 1.2, 5, 25, 50 and 100 mg/L
- Sampling schedule:
Control: at 0 and 48 hours
Test concentration: at 0 and 48 hours

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct weighings were prepared to give the desired test concentrations. To achieve this
the test substance was added to 1 litre of dilution water for each test concentration and treated, with an ultra tunax for 60 seconds at 3000 rpm.
- Controls: Yes
- Test concentration separation factor: 2
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: Strain of Bundesgesundheitsamt Berlin
- Stage and instar at study initiation: neonates
- Method of breeding: parthenogenetic females
- Source: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility.
- Age of parental stock: maintained since more than 15 years in the test facility.
- Feeding during test: no feeding during the exposure period

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

15.6 °dH (= 278.5 mg/L CaCO3)

Test temperature:

19.9 - 20 °C

pH:

7.9

Dissolved oxygen:

94 - 99 %

Nominal and measured concentrations:

Nominal = 6.3, 12.5, 25, 50 and 100 mg/L
Mean Measured = 2, 7, 13, 38 and 78 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Karlsruher bottles holding 10 neonates in 250-300 mL of test medium
- open or closed: closed bottle conditions
- Volume of solution: 250-300 mL
- Aeration: none
- No. of organisms per vessel: 10 neonates per vessel
- No. of vessels per concentration: 2 replicates per concentration
- No. of vessels per control: 2 replicates per control

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water (so-called 'M4 medium', originally described in Water Research 24 (9), September 1990: 1157-1167), prepared according to the recommendations of Bundesgesundheitsamt Berlin.
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark

EFFECT PARAMETERS MEASURED: Induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours

RANGE-FINDING STUDY
- Test concentrations: not reported
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

78 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

13 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Other biological observations: none
- Mortality of control: 0 %
- Immobilisation of control: 0 %

Reported statistics and error estimates:

Probit analysis

 

Validity criteria for the measurement of the fish toxicity

Target condition according to guideline:	Actual condition according to the study:	Validity criteria met:
In the control, including the solubilising agent, not more than 10 % of the daphnids should have been immobilized. (Not more than 10 % of the control daphnids should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water)	No immobilization observed in control	Yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/l in control and test vessels.	Dissolved oxygen concentration was atleast 8.5 mg/L in control and test vessels.	Yes
The nominal test concentrations and the result of all analyses to determine the concentration of the test substance in the test vessels; the recovery efficiency of the method and the limit of determination should be reported.	Adequate information was reported	Yes
The pH should not vary by more than 1 unit	The pH recorded was 7.9	Yes
There should be evidence that the concentration of the test substance has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the exposure. If the deviation from the nominal concentration is >20%, results should be based on the measured concentration (geometric mean in static and semi-static tests, arithmetic mean in flow-through tests; time weighted average, where applicable).	Measured concentration for all concentrations at 0 and 48 h were < 80 % of nominal concentration except the measured concentration of the highest concentration (100 mg/L) at 0 h. Nevertheless, the LC50 was based on the mean of the measured concentration during the 0 - 48 h exposure period.	Yes

 

Validity criteria fulfilled:

yes

Remarks:

See more details in the table in the section "Any other information on results incl. tables"

Conclusions:

The test substance affected the mobility of daphnia magna with EC0 =13 mg/L and EC100=78 mg/L (mean measured concentration).

Executive summary:

The 48 h-acute toxicity of the test substance to Daphnia magna was studied under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia'. Test species were exposed to control and test chemical at nominal concentrations of 6.3, 12.5, 25, 50 and 100 mg/L (mean measured concentrations: 2, 7, 13, 38 and 78 mg/L) for 48 h. Immobilisation and locomotion effects were monitored daily. Analysis of the immobilisation rates (by probit analysis) resulted in 48 h EC0 and EC100 of 13 and 78 mg/L, respectively. The results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 32 - 82% of nominal values at 0 hours, and from 32 - 76% of nominal values at 48 hours, respectively.

Description of key information

The 48 h-acute toxicity of the test substance to Daphnia magna was studied under static conditions. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (OJ. No. L383A, 29.12.92) Part C, Method 2 'Acute toxicity for Daphnia'. Test species were exposed to control and test chemical at nominal concentrations of 6.3, 12.5, 25, 50 and 100 mg/L (mean measured concentrations: 2, 7, 13, 38 and 78 mg/L) for 48 h. Immobilisation and locomotion effects were monitored daily. Analysis of the immobilisation rates (by probit analysis) resulted in 48 h EC0 and EC100 of 13 and 78 mg/L, respectively. The results are expressed in terms of mean measured concentrations. Measured concentrations ranged from 32 - 82% of nominal values at 0 hours, and from 32 - 76% of nominal values at 48 hours, respectively.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 13 mg/L