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Diss Factsheets
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EC number: 932-286-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May, 27, 1988-July 1, 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with restrictions (analytical purity not reported; only 4 strains tested)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- analytical purity not reported; only 4 strains tested
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,2,4,6,6-pentamethylheptane-4-thiol
- EC Number:
- 296-714-7
- EC Name:
- 2,2,4,6,6-pentamethylheptane-4-thiol
- Cas Number:
- 93002-38-1
- Molecular formula:
- C12H26S
- IUPAC Name:
- 2,2,4,6,6-pentamethylheptane-4-thiol
- Details on test material:
- - Name of test material (as cited in study report): TDM (tertiar dodecylmercaptan)
- Physical state: clear colourless liquid
- Analytical purity: not reported
- Lot/batch No.: 27051988
- Storage condition of test material: at room temperature
Constituent 1
Method
- Target gene:
- his operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: The strains are deep rough (i.e. partly deficient in lipopolysaccharide side chains of their cell walls) and UV repair ability is reduced.
- Metabolic activation:
- with and without
- Metabolic activation system:
- Mammalian metabolism is simulated by S9 mix, which is obtained from livers of at least six adult male Sprague Dawley rats, treated with Aroclor 1254 (500 mg/kg body weight, 5 days before the sacrifice) together with cofactors.
- Test concentrations with justification for top dose:
- The following doses were evaluated in the first test:
1. Negative control 0 µg/plate;
2. TDM 12500 µg/plate;
3. TDM 2500 µg/plate;
4. TDM 500 µg/plate;
5. TDM 100 µg/plate;
6. TDM 20 µg/plate.
Doses 3 to 6 were produced by progressively diluting the highest dose (2) at 1:5 with the solvent.
Due to the substance´s toxicity the doses chosen for the repeated tests ranged from 300 µg to 9600 µg per plate. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: ethanol for TDM; DMSO for positive controls
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Migrated to IUCLID6: -S9, tested at 10 µg/plate; only TA 1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: nitrofurantoin (-S9, tested at 0.2 µg/plate; only TA100)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-1,2-phenylene diamine (-S9, tested at 10 µg/plate, only TA 1537)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-1,2-phenylene diamine (-S9, tested at 0.5 µg/plate, only TA 98)
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- ethanol
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (+S9, tested at 3 µg/plate, all strains)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 hours
- Selection time (if incubation with a selection agent): 48 hours
SELECTION AGENT (mutation assays): L-histidine
DETERMINATION OF CYTOTOXICITY
- Method: The toxicity of the substance was assessed in 3 ways. First, the background growth on the plates for the mutant determination was grossly appraised. Secondly, a toxic effect of the substance was assumed when the mutant count per plate was clearly lower than a negative control count in dose correlation. Thirdly, the titer was determined. - Evaluation criteria:
- A reproducible and dose-related increase in mutant counts for at least one strain is considered positive. For TA 1535, TA 100, and TA 98 in principle a twofold increase compared to the corresponding negative controls should be reached, whereas for TA 1537 at least a threefold increase should be reached. Otherwise, the result is evaluated as negative.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- There was an indication of a bacteriotoxic effect of TDM at all doses. The total bacteria counts consistently produced results smaller than the negative controls. In addition, an inhibition of growth was noted.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
Maximum number of revertants (means ± SD):
Trial 1 (20 - 12500 µg/plate) |
||||
|
Control |
Test Substance (µg/plate) |
||
Strain |
-S9 |
+S9 |
-S9 |
+S9 |
TA1535 |
10 |
19 |
10 (2500) |
16 (20) |
TA100 |
53 |
79 |
57 (20) |
74 (20) |
TA1537 |
8 |
6 |
7 (100) |
6 (20) |
TA98 |
24 |
29 |
19 (100) |
35 (2500) |
Trial 2 ( 0- 9600 µg/plate) |
||||
TA1535 |
13 |
17 |
12 (300) |
15 (300) |
TA100 |
79 |
78 |
65 (300) |
63 (600) |
TA1537 |
8 |
8 |
8 (300; 9600) |
6 (600, 2400) |
TA98 |
16 |
34 |
22 (600) |
32 (9600) |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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