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Diss Factsheets
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EC number: 608-591-2 | CAS number: 31221-06-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
Diazobarbitursäure was initially investigated according to OECD TG 471 using the Salmonella/microsome plate incorporation methodology and Salmonella typhimurium TA1535, TA100, TA1537, TA98 and TA102. The test item was dissolved in DMSO, in doses of up to and including 5000 µg/plate. In all of the Salmonella typhimurium strains in test a biologically relevant increase in the mutant count compared to the corresponding negative control was found. Due to these results Diazobarbitursäure has to be regarded as mutagenic in the Salmonella/Microsome test.
In the available HPRT test in mamalian V79 cells of the Chinese hamster which was performed according to OECD TG 476 and GLP, concentrations up to 1600 µg/mL were used. No substantial and reproducible dose dependent increase in the mutation frequency was observed in both main experiments, therefore, Diazobatrbiturc acid is considered to be non-mutagenic in this HPRT assay.
Overall, there are two studies available investigating point mutations. The Ames test in bacteria yielded a positive result, whereas the HPRT test using somatic mammalian cell sytem, was negative. Both test are performed according to the respective guideline under GLP conditions and are evaluated with Klimisch score 1. Based on the negative data in the mamalian cell assay (HPRT) it can be concluded that mutagenicity of the test item is unlikely.
Short description of key information:
There are two studies available investigating point mutations. The Ames test in bacteria yielded a positive result, whereas the HPRT test using somatic mamalian cells was negative. Overall, based on the available data, mutagenicity of the test item is unlikely.
Endpoint Conclusion:
Justification for classification or non-classification
Overall, there are two studies available investigating point mutations. The Ames test in bacteria yielded a positive result, whereas the HPRT test using somatic mammalian cell sytem, was negative. Both test are performed according to the respective guideline under GLP conditions and are evaluated with Klimisch score 1. Based on the negative data in the mamalian cell assay (HPRT) it can be concluded that mutagenicity of the test item is unlikely.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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