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EC number: 700-408-5 | CAS number: 103429-90-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2019-04-17 to 2019-04-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of the test item was prepared at a concentration of 10 g/L. Bottles were prepared with deionized water, synthetic sewage feed and the test was performed at 3 different concentrations of the test item (1000 mg/L, 100 mg/L and 10 mg/L). pH of the solutions was checked. The mud was added before starting the test.
- Controls: Blanks were used which contained deionized water and an equal volume of activated sludge and synthetic medium (without reference substance and test item). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected:
San Rocco near Monza
- Method of cultivation: Activated sludge was collected and used on day of collection.
- Preparation of inoculum for exposure: No coarse particles were present so settling was not necessary. Then the mud samples were washed and analyzed to prepare the inoculum before their use. The sludge was continuously aerated at the test temperature.
- Initial biomass concentration: The suspended solids in the mud solution was 3 g/L to have a final mixture concentration of 1.5 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.5 ± 0.5
- Nominal and measured concentrations:
- Nominal: 1000 mg/L, 100 mg/L and 10 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass bottles
- Material, size, headspace, fill volume: Glass
- Aeration: The vessels were aerated to keep the dissolved oxygen concentration above 60-70% saturation and to maintain the sludge flocs in suspension.
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per abiotic control (replicates): 3
- Sludge concentration (weight of dry solids per volume): The suspended solids in the mud solution was 3 g/L to have a final mixture concentration of 1.5 g/L.
- Nutrients provided for bacteria: Synthetic sewage feed
- Nitrification inhibitor used (delete if not applicable): None
OTHER TEST CONDITIONS
- Adjustment of pH: The pH of the reference substance stock solution was adjusted to pH 7.45 with NaOH (1 M).
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Respiration rate
TEST CONCENTRATIONS
- Range finding study : Only range finding study was conducted since results were already sufficient.
- Test concentrations: 1000 mg/L, 100 mg/L and 10 mg/L
- Results used to determine the conditions for the definitive study: No definitive study necessary - Reference substance (positive control):
- yes
- Remarks:
- 3,5 Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Inhibition of oxygen uptake
- Details on results:
- - Blank controls oxygen uptake rate:
33.62 mg oxygen per one gram of activated sludge in an hour.
- Coefficient of variation of oxygen uptake rate in control replicates: 9.17% - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50 of 3,5 – Dichlorophenol: 5.71 mg/L for total respiration - Validity criteria fulfilled:
- yes
- Conclusions:
- The activated sludge respiration inhibition test was conducted in compliance with GLP and in accordance with OECD 209. All validity criteria were met and no deviations were reported. The EC50 value of the test item "3-Methoxy-3-methylbutyl acetate (MMB-AC)” after 3 hours was determined to be greater than 1000 mg/L.
Reference
Table 1: Mean percent inhibition total respiration rate of the test item
Concentration |
% of inhibition total respiration rate Test item “3-Methoxy-3-methylbutyl acetate (MMB-AC)” |
10 mg/L |
0.46 |
100 mg/L |
1.32 |
1000 mg/L |
4.43 |
Table 2: Mean percent inhibition total respiration rate of the reference substance.
Concentration |
% of inhibition total respiration rate Reference substance “3,5 - Dichlorophenol” |
1 mg/L |
19.37 |
10 mg/L |
61.60 |
50 mg/L |
85.19 |
Description of key information
The activated sludge respiration inhibition test was conducted in compliance with GLP and in accordance with OECD 209. All validity criteria were met and no deviations were reported. The EC50 value of the test item "3-Methoxy-3-methylbutyl acetate (MMB-AC)” after 3 hours was determined to be greater than 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
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