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EC number: 227-575-2 | CAS number: 5894-60-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
The synthetic sewage feed (16 ml) and an adequate amount of the test substance loading were mixed and made up to 250 ml with Milli-RO water in a 1 litre bottle. The pH was determined. Thereafter 250 ml activated sludge was added. This was the start of the test. The mixture was then aerated during the contact time, using a pipette as an aeration device. - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: Municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Preparation of inoculum for exposure: The sludge was coarsely sieved, washed and diluted with ISO-medium. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/l of sludge, as used for the test). The pH was 7.7 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic sewage feed was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Test temperature:
- 18.7 and 22.0°C
- pH:
- 7.5 ± 0.5 (7.3-7.5)
- Nominal and measured concentrations:
- In a combined limit/range-finding test loading rates of 10, 100 and 1000 mg/l were tested.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material: All glass bottles/vessels.
- Aeration: yes
- No. of vessels per concentration (replicates): The highest loading rate was tested in triplicate, lower concentrations consisted of one replicate. In addition, blankcontrols and a nitrification control of the sludge were included.
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Adjusted ISO medium, formulated using RO-water (tapwater purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with
the following composition:
CaCl2.2H2O 211.5 mg/l
MgSO4.7H2O 88.8 mg/l
NaHCO3 46.7 mg/l
KCl 4.2 mg/l
OTHER TEST CONDITIONS
- Adjustment of pH: Since, it is preferred that test solutions have a neutral pH, the pH was adjusted to pH 7.5 ± 0.5 with 1 N NaOH.
EFFECT PARAMETERS MEASURED : The influence of Trichloro(hexadecyl)silane on the respiration rate of activated sludge was investigated
after a contact time of 3 hours. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Reported statistics and error estimates:
- the combined limit/range-finding test no statistically significant inhibition of the respiration rate of the
sludge was recorded at and below a loading rate of 1000 mg Trichloro(hexadecyl)silane per litre (Two
Sample t-Test : α=0.05 Toxstat - Validity criteria fulfilled:
- yes
Reference
Under the conditions of this present test Trichloro(hexadecyl)silane was not toxic to waste water
(activated sludge) bacteria at or below a loading rate of 1000 mg/l (NOEC).
Description of key information
Toxicity to microorganisms: 3-h EC50 >1000 mg/L; NOEC >= 1000 mg/L (OECD 209)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The influence of trichloro(hexadecyl)silane on the respiration rate of activated sludge was investigated after a contact time of 3 hours. The study procedures described in this report were based on OECD 209. In a combined limit/range-finding test loading rates of 10, 100 and 1000 mg/L were tested. No statistically significant inhibition of the respiration rate of the sludge was recorded at and below a loading rate of 1000 mg trichloro(hexadecyl)silane per litre. Furthermore, there was no oxygen uptake from abiotic processes and the results at 1000 mg/L with a nitrification inhibitor showed that the heterotrophic inhibition of the respiration rate was in general comparable to the total inhibition. In conclusion, trichloro(hexadecyl)silane was not toxic to waste water (activated sludge) bacteria at or below a loading rate of 1000 mg/L (NOEC).
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