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EC number: 252-021-1 | CAS number: 34432-92-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8.10. – 17.10. 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study was carried out in accordance with internationally valid GLP principles.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- yes
- Remarks:
- (see Overall remarks)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- TEST SYSTEM
The activated biological sludge containing mixed culture of microorganisms obtained from the second step of sewage treatment plant of Pardubice was used for the testing. The wastewater processed by the sewage treatment plant is predominantly municipal.
PREPARATION OF THE INOCULUM
The activated sludge was collected two days before the day of testing. After the sample collection the sludge was washed with potable water for 0.5 hour and subsequently decanted for 0.5 hour. This procedure was repeated three times in total.
Further the sludge was modified by addition of 50 mL of cultivation medium per 2 L of diluted sludge suspension at permanent aeration till the day of test.
The dry weight was determined from 10 mL of sludge suspension after 0.5 hour sedimentation.
Before the test the sludge was suspended in water up to concentration about 4 000 mg of sludge dry weight per litre. The pH adjustment to 6.0 was carried out. In that way modified sludge suspension was aerated until the use.
JUSTIFICATION FOR THE SELECTION OF THE TEST SYSTEM
The activated sludge used in the test is in conformity with the recommendations of the test guidelines. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- pH:
- approx. 6 – 8
- Nominal and measured concentrations:
- 100, 180, 320, 560 and 1000 mg.L-1 (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: oxygen vessels (BOD bottles) with volume of about 280 mL for measurement of oxygen concentration
- Type (delete if not applicable): closed
- Aeration: by filtered pressured air
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Sludge dose on concentration of 4000 mg.L-1 : 303 mL.L-1
WATER PARAMETERS
- deionized water
OTHER TEST CONDITIONS
- Adjustment of pH: was not carried out
- Photoperiod: daily lighting
TEST PROCEDURE
Defined dose of the test substance or reference substance , 100 mL of deionized water, 16 mL of the synthetic nutrient medium and 200 mL of the prepared sludge suspension were introduced into each test container. The volume was replenished up to 500 mL with deionized water.
The control samples were prepared only from the same volume of nutrient medium and sludge and replenished up to 500 mL with water.
The check of abiotic decomposition was performed at the highest concentration of the test substance and nutrient medium without sludge and with addition of 1 mL of mercury chloride solution.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): The decline of oxygen concentration was measured and recorded at minimal 10-minute intervals (respiration rate of activated sludge)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8 (100, 180, 320, 560 and 1000 mg.L-1 )
- Range finding study
- Test concentrations: 46, 100, 220, 460 and 1 000 mg.L-1 (spacing factor 2.2)
- The preliminary test was performed to determine the test substance concentrations to be used in the main test. - Reference substance (positive control):
- yes
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 = 8.2 mg.L-1 (95% confidence interval: 6.2 – 9.9 mg.L-1) - Validity criteria fulfilled:
- yes
- Conclusions:
- On the basis of the effective concentrations describing the respiration inhibition of activated sludge caused by the test substance, Solvent Yellow 124, the EC50 was found to be:
EC50 > 1 000 mg.L-1 - Executive summary:
The influence of the test substance, Solvent Yellow 124, on the respiration rate of activated sludge was investigated after a contact time of 3 hours.
Test was performed according to method C.11 - Activated Sludge Respiration Inhibition Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The preliminary test was performed using 5 concentrations in range 46 – 1 000 mg.L-1 with a geometric factor of 2.2 to determine the concentrations to be used in the main test.
In the main test, 5 concentrations of the test substance in geometric progression with factor of 1.8 (100, 180, 320, 560, 1000 mg.L-1) were used together with two control experiments (without the test substance), one at the beginning and one at the end of the test.
3,5-dichlorophenol was used as reference substance. Five concentrations in geometric progression with a factor of 1.4 (5.2, 7.3, 10, 14 and 20 mg.L-1) were selected.
The abiotic decomposition was not detected even at the highest concentration of the test substance.
The pH value during the main test was in the range of 7.0 to 7.8.
Results of the respiration inhibition test
On the basis of the effective concentrations describing the respiration inhibition of activated sludge caused by the test substance, Solvent Yellow 124, the EC50 was found to be:
EC50 > 1 000 mg.L-1
Under the current experimental conditions, all tested concentrations in the main test caused no significant inhibition of respiration rate.Therefore it was not possible to calculate the EC50 value.
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
On the basis of the effective concentrations describing the respiration inhibition of activated sludge caused by the test substance, Solvent Yellow 124, the EC50 was found to be:
EC50 > 1 000 mg.L-1
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