Methyl DL-serinate hydrochloride

Water solubility

Administrative data

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28-29 Oct 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Principles of method if other than guideline:

As the water solubility was high, the flask method was used in a modified version. The water solubility of the test item is indicated as a minimum value.

GLP compliance:

yes (incl. QA statement)

Remarks:

Hess. Ministerium für Umwelt, ländlichen Raum und Verbraucherschutz, Mainzer Str. 80, 65189 Wiesbaden, Germany

Type of method:

flask method

Test material

Test material information

Constituent 1

Reference substance name:

Methyl DL-serinate hydrochloride

EC Number:

227-047-1

EC Name:

Methyl DL-serinate hydrochloride

Cas Number:

5619-04-5

Molecular formula:

C4H9NO3.ClH

IUPAC Name:

methyl 2-amino-3-hydroxypropanoate hydrochloride

Details on test material:

- Name of test material (as cited in study report): DL-Serinmethylester-HCl
- Chemical name: (RS)-2-Amino-3-hydroxy-propionic acid methylester hydrochloride
- Appearance: white solid
- Expiration date of the lot/batch: 11 Jun 2013
- Storage condition of test material: In original container, at room temperature (10 to 30 °C), in the dark

Results and discussion

Water solubility

Water solubility:

> 588 g/L

Temp.:

20 °C

Remarks on result:

other: no pH reported; temperature given as approx. 20 °C

Any other information on results incl. tables

Result of the preliminary test:

The water solubility of the test item was estimated to be higher than 1 g/L.

Result of the main test:

The concentration of the test item was measured using an HPLC method. The linearity of the analytical method was proven in the concentration range from 100 to 1000 mg test item/L. The regression coefficient was determined to be 0.9996. The results are shown in the table.

Sample description	Concentration found [mg/L]*)	Dilution factor	Concentration calculated [g/L]
Replicate 1	284.899	2000	569.797
Replicate 2	303.453	2000	606.906
Mean value (n=2)			588
Standard dev. (n=2)			26

*) Concentration found - rounded value calculated from the regression data.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): very soluble (> 10000 mg/L)
The water solubility of DL-Serinmethylester-HCI was calculated to be higher than 588 g/L (mean value, 26 g/L standard deviation) at approx. 20 °C.

Executive summary:

The water solubility of the test item was determined using the flask method in a modified version. The concentration of the test item was determined using HPLC method. The linearity of the analytical method was proven in the concentration range from 100 to 1000 mg test item/L. The regression coefficient was determined to be 0.9996.