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EC number: 205-238-0 | CAS number: 136-30-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 798.5265 (The Salmonella typhimurium Bacterial Reverse Mutation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Sodium dibutyldithiocarbamate
- EC Number:
- 205-238-0
- EC Name:
- Sodium dibutyldithiocarbamate
- Cas Number:
- 136-30-1
- Molecular formula:
- C9H19NS2.Na
- IUPAC Name:
- sodium (dibutylcarbamothioyl)sulfanide
- Details on test material:
- - Physical state: straw-coloured liquid
- Analytical purity: 47.5% aqueous solution of density 1.086 g/ml
- Lot/batch No.: 8905275
- Storage condition of test material: kept at room temperature, in the original container and protected from light.
Constituent 1
Method
- Target gene:
- DNA base pairs
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- derived from rat liver (S-9 mix)
- Test concentrations with justification for top dose:
- 50, 158, 500, 1580 and 5000 µg per plate (for 47.5% aqueous solution of the substance), corresponding to 23.75, 75.05, 237.5, 750.5 and 2375 µg per plate for pure (anhydrous) substance
- Vehicle / solvent:
- - Solvent used: distilled water
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Remarks:
- with and without S-9
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Incubation period: 2 days
NUMBER OF REPLICATIONS: Triplicate
DETERMINATION OF CYTOTOXICITY
- Method: examination for the presence of a background lawn of non-revertant colonies - Evaluation criteria:
- No data
- Statistics:
- No data
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- CYTOTOXICITY:
The lowest level of SDBC causing visible thinning of the background lawn of non-revertant cells was 5000 µg/plate. This was therefore selected as the top exposure level for use in the main assays.
Any other information on results incl. tables
Summary of the revertant colony means:
Plate N° |
Addition (µg) |
S-9mix: + present; - absent |
Revertant colony means |
||||||||
TA98 (Test 1) |
TA98 (Test 2) |
TA100 (Test 1) |
TA100 (Test 2) |
TA1535 (Test 1) |
TA1535 (Test 2) |
TA1537 (Test 1) |
TA1537 (Test 2) |
||||
1 |
None; sterility check |
|
+ |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2 |
SDBC sterility check |
5000 |
- |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
3 |
SDBC |
5000 |
+ |
14 |
24 |
29 |
28 |
6 |
7 |
8 |
6 |
4 |
SDBC |
1580 |
+ |
28 |
33 |
70 |
55 |
14 |
12 |
7 |
7 |
5 |
SDBC |
500 |
+ |
34 |
32 |
108 |
109 |
15 |
13 |
7 |
7 |
6 |
SDBC |
158 |
+ |
38 |
32 |
111 |
108 |
15 |
13 |
7 |
6 |
7 |
SDBC |
50 |
+ |
36 |
33 |
113 |
111 |
14 |
14 |
7 |
7 |
8 |
Distilled water |
|
+ |
37 |
32 |
110 |
110 |
13 |
13 |
8 |
7 |
9 |
SDBC |
5000 |
- |
18 |
29 |
109 |
109 |
13 |
12 |
5 |
8 |
10 |
SDBC |
1580 |
- |
29 |
34 |
109 |
111 |
14 |
14 |
8 |
7 |
11 |
SDBC |
500 |
- |
33 |
35 |
112 |
111 |
15 |
15 |
8 |
6 |
12 |
SDBC |
158 |
- |
34 |
31 |
112 |
111 |
14 |
15 |
7 |
8 |
13 |
SDBC |
50 |
- |
33 |
34 |
112 |
111 |
14 |
16 |
7 |
7 |
14 |
Distilled water |
|
- |
36 |
31 |
110 |
111 |
14 |
16 |
8 |
8 |
15 |
Benzo(a)pyrene |
|
- |
31 |
26 |
104 |
102 |
12 |
16 |
6 |
6 |
16 |
Benzo(a)pyrene |
|
+ |
260 |
393 |
590 |
470 |
846 |
494 |
218 |
171 |
17 |
2-Nitrofluorene |
|
- |
440 |
909 |
531 |
673 |
644 |
416 |
167 |
146 |
18 |
None; 10E-6 dilution of bacterial culture only |
|
- |
115 |
113 |
115 |
113 |
114 |
116 |
114 |
113 |
Applicant's summary and conclusion
- Conclusions:
- Negative in the Ames test.
- Executive summary:
The ability of sodium dibutyldithiocarbamate (SDBC) in its manufactured form (as 47.5% aqueous solution) to induce gene mutations in bacteria was studied in Ames test with Salmonella typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 at test concentrations 50, 158, 500, 1580 and 5000 µg per plate (corresponding to 23.75, 75.05, 237.5, 750.5 and 2375 µg per plate for pure (anhydrous) substance), in the presence and absence of metabolic activation (Life Science Research Ltd, 1990). The study was performed in accordance with OECD Guideline 471 and EPA OTS 798.5265 and under GLP. The lowest level of SDBC causing visible thinning of the background lawn of non-revertant cells was 5000 µg/plate. The substance did not induce a statistically significant increase in the number of revertants in any strain, both with and without metabolic activation, indicating that sodium dibutyldithiocarbamate (SDBC) is not mutagenic in Ames test.
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