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Diss Factsheets
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EC number: 464-520-3 | CAS number: 189813-45-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with analytical monitoring
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): 520 F - Chlormethyloximether
- Physical state: solid/ dark green
- Analytical purity: 94.4 g / 100 g
- Batch No.: 31196-182
- Storage condition of test material: ambient temperature
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test concentrations were prepared by dilution of a stock dispersion. The concentration of the stock dispersion was 111.1 mg/L. The test substance was weighed in and treated by ultra sonification for 30 minutes. The stock dispersion was a dispersion with fine particles, which were homogenously distributed, so that the stock dispersion could be used to prepare the test batches.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: unicellular green alga
- Strain: SAG 86.81
- Source (laboratory, culture collection): obtained at regular intervals from SAG (Collection of algal cultures in Göttingen) and kept in liquid culture in the Laboratory of Experimental Toxicology and Ecology
- Age of inoculum (at test initiation): 3 days
PRE CULTURE
- Seed-culture: A seed culture was incubated for 4 days at 23 °C ± 2 °C
- Final cell density: 150E+04 cells/ml
- Pre-culture: Seed-culture was taken to inoculate a pre-culture (initial cell density: 1 x 104 cells/mL). The pre-culture was
incubated for 3 days at 23 °C ± 2 °C.
- Final cell density: 92E+04 cells/ml
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21 - 25 °C
- pH:
- uninoculated (after 0 h): 7.9 - 8.2
uninoculated (after 72 h): 7.6 - 8.1
inoculated (after 72 h): 7.6 - 9.6 - Nominal and measured concentrations:
- Nominal test concentrations: control, 0.391, 0.781, 1.56, 3.13, 6.25, 12.5, 25, 50, 100 mg/L
Mean measured concentrations: -, 0.163, 0.438, 0.955, 1.57, 2.65, 5.76, 14.9, 32.2, 63.7 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: Erlenmeyer flasks (nominal volume 250 mL) plugged with gas permeable siliconsponge caps, 250 ml, 100 ml
- Initial cells density: 1E+04 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement: temperature was continuously measured during the whole test period in the climate
chamber; pH in additional uninoculated parallels: after 0 h in parallel 5 (this parallel was disposed of after measurement), after 72 h in parallel 6 and in the inoculated parallel 1 of each concentration.
OTHER TEST CONDITIONS
- Photoperiod: permanent illumination
- Light intensity and quality: Artificial light, type universal white, about 6000 – 10000 Lux at a wave length of 400 - 700 nm
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: after 72 h in a counting chamber (Neubauer improved) in replicate No. 2 of the inoculated control
- Chlorophyll measurement: In vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm) after 0, 24, 48 and 72 h
TEST CONCENTRATIONS
- Range finding study: yes
- Results used to determine the conditions for the definitive study: EC50 range derived from 72 hour range finding: 10 - 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 14.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 32.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 10.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 29.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Results with reference substance (positive control):
- The ErC50 (72 h) of the control substance potassium dichromate was 0.880 mg/L (Date of the last control experiment: 4 Apr 2006
Any other information on results incl. tables
For an evaluation of the results the nominal concentrations should be used, since the effects observed in this test include effects of undissolved test substance of products of hydrolysis.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.