PIGMENTADDITIV RL

Endpoint summary

Additional information

The submission substance is highly insoluble in water (3.9 µg/L, see IUCLID section 4). Owing to this, (super)saturated solutions were tested with nominal concentrations exceeding the maximum water solubility of the submission substance by far. In the available tests on fish (acute) and Daphnia magna (acute), no toxic effects were observed. Data on aquatic plants and microorganisms are available for the read across substance C.I. Pigment Violet 23.

 

Freshwater fish:

Neither mortality nor clinical signs were observed in zebra fish exposed to a nominal test concentration of 500 mg/L during the 96-hours testing period. As the test substance is highly insoluble in water, concentration levels toxic for zebra fish could not be reached. Therefore, the 96h-LC50 for zebra fish was far beyond the maximum solubility of the test substance and with regard to the nominal concentration tested the LC50 was determined to be > 500 mg/L.

Freshwater invertebrates:

During the 48-hours testing period, no immobilization of Daphnia magna occurred in the two lowest test concentration, i.e. 1 mg/L and 1.8 mg/L. After 24 hours, precipitation of test item particles occurred at all test concentrations. At higher test concentrations undissolved particles adsorbed on the cuticle of the test organisms and hindered the motility of the organisms. Thus, observed immobility of Daphnia magna was related to the physical impact of undissolved particles instead of an inherent toxicity of the test substance. The derived ECx-values were based on nominal concentrations, i.e. 1.8 mg/L and > 500 mg/L for the 48h-EC0 and 48h-EC50, respectively.

The toxicity of the read across substance C.I. Pigment Violet 23 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201. A limit test with a saturated solution (nominal concentration 100 mg/L) was carried out. In this study the test item (C.I. Pigment Violet 23) did not cause any inhibiting effects to Desmodesmus subspicatus after 72 h.

A respiration inhibition test with activated sludge according to OECD guideline 209 was performed with the read across substance C.I. Pigment Violet 23. The test was performed under static conditions with the nominal limit concentration of 1000 mg/L. The test item is not toxic at concentrations ≤ 1000 mg/L to activated sludge of a municipal sewage treatment plant.