1H-Imidazole, hydrobromide (1:1)

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Remarks:

Toxic effect on specific growth rate

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

not speciifed

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Comparison of the susceptibility of 11 freshwater species to 8 chemical compounds. II. (semi)chronic toxicity tests

Author:

Slooff, W.; Canton, J.H.

Year:

1983

Bibliographic source:

Aquatic Toxicol. 4, 271-281

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The toxicity test with bacteria were carried out with standardised laboratory cultures of Pseudomonas fluorescens. Therefore, the toxic effects of sodium bromide on the specific growth rate was investigated under distinct parameters.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Sodium bromide

EC Number:

231-599-9

EC Name:

Sodium bromide

Cas Number:

7647-15-6

Molecular formula:

BrNa

IUPAC Name:

sodium bromide

Test material form:

not specified

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

Not applicable.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: dilution in test medium
- Differential loading: not specified

Test organisms

Test organisms (species):

Pseudomonas fluorescens

Details on inoculum:

- Laboratory culture: yes, standardised laboratory culture
- Initial biomass concentration: ca. 10E10 organisms per group in 3-fold

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

8 h

Test conditions

Test temperature:

22 ± 2 °C

Nominal and measured concentrations:

not specified, but at least up to the NOEC of 3.2 g/L were tested.

Details on test conditions:

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
The medium for bateria contains 0.2 g/L MgSO4 x 7 H20, 10 g/L K2HPO4, 0.5 g/L l-arginine, 2 g/L citric acid, 5 g/L glucose, 1 g/L NH4Cl, 3.5 g/L NaNH4HPO4 x H2O, 2 mg/L histidine, 0.5 mg/L biotine.

OTHER TEST CONDITIONS
- Photoperiod: darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
specific growth rate

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

8 h

Dose descriptor:

NOEC

Effect conc.:

3 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Results with reference substance (positive control):

Not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The test item was not found to inhibit the specific growth rate of Pseudomonas flurescens at a concentration ≥3200 mg/L in a 8 hour static test in darkness. Therefore, the 8-h NOEC value for inhibition of specific growth rate was determined to be ≥3.2 g sodium bromide/L.