Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 266-096-3 | CAS number: 66063-05-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-01-17 to 2001-01-24
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 1988
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: direct weighing
- Controls: Two controls without test substance are included in the test design, one at the start and the other at the end of the test series. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: mixed population of aquatic microorganisms (activated sludge).
- Name and location of sewage treatment plant where inoculum was collected: aeration tank of a waste water treatment plant treating predominantly domestic sewage (Wupper area water authority).
- Pretreatment: aeration of the activated sludge; daily feed with synthetic medium.
- pH of the suspension before application: 6.8 - Test type:
- static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.6 - 19.8°C
- pH:
- 7.4 - 8.1
- Dissolved oxygen:
- Test start: 5.2 - 6.3 mgO2/L ; test end: 2.6 - 3.2 mgO2/L.
physicochemical oxygen consumption: 8.4 - Nominal and measured concentrations:
- Nominal concentration of 100, 1000, and 10000 mg a.s./L.
- Details on test conditions:
- TEST SYSTEM
- Test substance concentration in physico-chemical oxygen consumption control (mg/L): 1000 mg/L
- Type of application: direct weighing
- Test concentration of the activated sludge: 480 mg/L suspended solid
- Incubation time: 3 hours with permanent aeration. - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.3 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: based on test substance aqueous solubility.
- Details on results:
- The pH varied between 8.0 and 8.1. The physicochemical oxygen demand was determined at the highest test concentration at a pH of 7.4.
The control respiration rates differed less than 15%. No physicochemical oxygen consumption was observed in the highest test concentration. Therefore, the 3h-EC50 for respiration inhibition of activated sludge was reported to be >10000 mg test substance/L.
The test concentrations were above the water solubility of the active substance (0.3 mg/L at 20°C). It must be assumed that the amount of test substance dosed above the aqueous solubility of the active ingredient is not bio-available and does not contribute to the exposure of the activated sludge to the test substance. Therefore, it is concluded that the 3h-EC50 for respiration inhibition of activated sludge is >0.3 mg a.s./L. - Results with reference substance (positive control):
- The 3h-EC50 of the reference substance 3, 5- dichlorophenol was within the range of 5 - 30 mgl/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- Respiratory rate of the 2 controls differs less than 15%, respiratory rate of the controls <60 mg 02 /L * h and EC50 of the reference substance 3.5-dichlorophenol is in the range 5 - 30 mg/L.
- Conclusions:
- The test concentrations were above the water solubility of the active substance (0.3 mg/L at 20°C). It must be assumed that the amount of test substance dosed above the aqueous solubility of the active ingredient is not bio-available and does not contribute to the exposure of the activated sludge to the test substance. Therefore, it is concluded that the 3h-EC50 for respiration inhibition of activated sludge is >0.3 mg a.s./L.
- Executive summary:
A study was performed to assess the toxicity of pencycuron (NTN 19701) to bacteria. The activated sludge was exposed to pencycuron (NTN 19701) at concentrations of 100, 1000 and 10000 mg a.s./L. The respiration rate of each mixture was determined after aeration periods of 3 hours. Pencycuron (NTN 19701) showed 8.2% respiration inhibition of activated sludge at a test item concentration of 10000 mg a.s./L. The 3h-EC50 for respiration inhibition of activated sludge was >0.3 mg a.s./L.
Reference
See "Attachments" in "Overall remarks, attachments" for the result tables.
Description of key information
A respiration inhibition study was performed to investigate the toxicity of pencycuron on activated sludge exposed to it for 3 hours. The EC50 was determined to be >0.3 mg/L.
Test species | Result | Assessment | Reference |
Activated sludge | 3-h EC50 >0.3 mg a.s./L | Key study | Müller (2001) |
Key value for chemical safety assessment
- EC50 for microorganisms:
- 0.3 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.