Benzoic acid, 3-amino-5-[[(2,3-dihydroxypropyl)amino]carbonyl]-2,4,6-triiodo-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

1989

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

adopted 4 Apr 1984

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

rac-5-Amino-N-(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamic acid

EC Number:

601-093-6

Cas Number:

111453-32-8

Molecular formula:

C11 H11 I3 N2 O5

IUPAC Name:

rac-5-Amino-N-(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamic acid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Schering AG
- Age at study initiation (mean and range, SD): 0-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

20.9 - 21.5 °C

pH:

7.5 - 8.7

Dissolved oxygen:

8.2 - 9.0 mg/L

Nominal and measured concentrations:

nominal: control, 10 g/L

Details on test conditions:

TEST SYSTEM
20 Daphnia were used for the test concentration and also for the control group, randomly distributed into groups of five. Labelled 100 ml test vessels were used and filled with 50 ml of the test solution and the dilution water (control group). The test was, performed with 4 replicates of each test solution and the dilution water control with 5 Daphnia in each vessel. One additional test vessel for the test concentration and the control group was used without Daphnia for the measurements of pH, temperature and oxygen content. The test started when the animals were added to the previously prepared test solution. The Daphnia were exposed to the test concentration and the control for a period of 48 hours under static conditions.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 10 g/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality of control: 0 daphnids
- Immobilisation of control: 0 daphnids
- Immobilisation of test item: 1 daphnid

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 (48 h) of Tamip monoamid (ZK 39.211) to Daphnia magna is higher than 10 g/L.

Executive summary:

Twenty daphnids were exposed to the test substance and a dilution water control for a period of 48 h. The test concentration in the limit test was 10 g/L. No abnormalities related to the exposure to the test compound were observed. The EC50 (48 h) was higher than 10 g/L.