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Diss Factsheets
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EC number: 446-560-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09 Jan - 16 Mar 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): secondary effluent of a domestic sewage treatment plant (Odenthal)
- Method of cultivation: aeration of mineral medium plus inoculum (filtrate) for 6 days
- Concentration of sludge: 5 mL/L
- Water filtered: Yes - Duration of test (contact time):
- 28 d
- Initial conc.:
- 18.3 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium
- Test temperature: 20 +/- 1 °C
- Aeration of dilution water: Aeration for 6 days
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: Glass bottles
- Number of culture flasks/concentration: 10 bottles
- Method used to create aerobic conditions: aeration of solution
- Measuring equipment: Dissolved oxygen was measured by chemical uptake
- Test performed in closed vessels due to significant volatility of test substance: Closed bottles
SAMPLING
- Sampling frequency: Every seven days
- Sample storage before analysis: No storage of samples
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes, 10 bottles
- Abiotic sterile control: No
- Toxicity control: Yes, 10 bottles
- Reference substance: Yes, 10 bottles - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 21 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 7 d
- Details on results:
- The toxicity control was degraded to 25% after 14 and 28 d, respectively. Thus, the test item is not toxic to the inoculum.
- Results with reference substance:
- 81 % degradation of reference substance over 28 days was observed.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results incl. tables'.
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test substance showed: 0 % degradation after 28 days. Therefore the test substance has to be classified as "Not Readily Biodegradable".
- Executive summary:
The test substance in a mineral medium was inoculated with secondary effluent and incubated for 28 d under aerobic conditions in completely full, closed bottles. During this period the biodegradation is followed by analysis of dissolved oxygen. The amount ofoxygen taken up by the test chemical, corrected for uptake by the blank inoculum run in parallel, is expressed as a percentage of theoretical oxygen demand (ThOD) or chemical oxygen demand (COD). The test substance showed: 0 % degradation after 28 days. Therefore the test substance has to be classified as "Not Readily Biodegradable". The reference compound showed 81 % degradation after 28 days.
Reference
Validity criteria for the measurement of the biodegradation according to OECD 301:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
In order to check the procedure, reference compounds which meet the criteria for ready biodegradability are tested by setting up an appropriate vessel in parallel as part of normal test runs. Suitable compounds are aniline (freshly distilled), sodium acetate and sodium benzoate. | Benzoic acid, sodium salt was used as a reference compound. | Yes |
A test is considered valid if the difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20% and if the percentage degradation of the reference compound has reached the pass levels by day 14. | % degradation of the two measured values of the test substance at the end of the test (28 d) range between 0 - 0% There was 81 % degradation of the test substance after 14 d - the pass level (60 % for ThOD) was reached. | Yes |
If in a toxicity test, containing both the test substance and a reference compound, less than 35% degradation (based on total DOC) or less than 25% (based on total ThOD or ThCO2) occurred within 14 days, the test substance can be assumed to be inhibitory. The test series should be repeated, using a lower concentration of test substance (if this can be done without seriously impairing the accuracy of the DOC determination) and/or a higher concentration of inoculum, but not greater than 30 mg solids/L. | The used concentrations of the test substance did not show toxic effects to bacteria (after 14, 21 and 28 d, 25 % degradation was measured based on ThOD). | Yes |
Description of key information
The test substance in a mineral medium was inoculated with secondary effluent and incubated for 28 d under aerobic conditions in completely full, closed bottles. During this period the biodegradation is followed by analysis of dissolved oxygen. The amount of oxygen taken up by the test chemical, corrected for uptake by the blank inoculum run in parallel, is expressed as a percentage of theoretical oxygen demand (ThOD) or chemical oxygen demand (COD). The test substance showed: 0% degradation after 28 days. Therefore, the test substance has to be classified as "Not Readily Biodegradable". The reference compound showed 81% degradation after 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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