Purine-2(3H),6(1H)-dione

Particle size distribution (Granulometry)

Link to relevant study record(s)

Reference

Endpoint:

particle size distribution (granulometry)

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 110 (Particle Size Distribution / Fibre Length and Diameter Distributions - Method A: Particle Size Distribution (effective hydrodynamic radius)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7520 (Particle Size, Fiber Length, and Diameter Distribution)

GLP compliance:

yes

Type of method:

sieving

Type of particle tested:

aggregate

Type of distribution:

mass based distribution

Specific details on test material used for the study:

Purity: > 99%

Key result

Percentile:

other: 0.5

Mean:

< 32 µm

St. dev.:

0

Key result

Percentile:

other: 5.5

Mean:

> 32 - <= 45 µm

St. dev.:

0.7

Key result

Percentile:

other: 22.6

Mean:

> 45 - <= 75 µm

St. dev.:

1.8

Key result

Percentile:

other: 29.3

Mean:

> 75 - <= 150 µm

St. dev.:

2

Key result

Percentile:

other: 29.3

Mean:

> 150 - <= 300 µm

St. dev.:

0.1

Key result

Percentile:

other: 11.2

Mean:

> 300 - <= 850 µm

St. dev.:

0.4

Key result

Percentile:

other: 1.5

Mean:

> 850 µm

St. dev.:

0

Conclusions:

The test substance was analyzed to estimate the geometric particle size. The average percent of particles larger than 850 µm was 1.5%. The average percent of particles in the range of 300 µm - 850 µm was 11.2%. The average percent of particles in the range of 150 µm - 300 µm was 29.3%. The average percent of particles in the range of 75 µm - 150 µm was 29.3%. The average percent of particles in the range of 45 µm - 75 µm was 22.6%. The average percent of particles in the range of 32 µm - 45 µm was 5.5%. The average percent of particles smaller than 32 µm was 0.5%.

Executive summary:

A known weight of the test substance was passed through six sieves with successively smaller mesh sizes. Specific mesh sizes were selected such that the first sieve allowed most or all of the material to pass through; the next four sieves allowed some of the material through while blocking some. The smallest sieve was small enough to block most or all of the material. The exact mesh sieve size numbers used were 20, 45, 50, 100, 200, and 400. Sieve openings were 850 µm, 300 µm, 150 µm, 75 µm, 45 µm and 32 µm, respectively. Each sieve was individually weighed and the weight recorded. The sieves were stacked from largest mesh (top) to smallest (bottom). A pan capable of holding and recovering material passing through the bottom sieve was placed beneath the smallest sieve. For each replicate, approximately 20 grams of test substance was weighed and placed on the largest sieve. Soft aggregates were broken up by gentle brushing, ensuring that no hard particles were crushed. Each sieve was agitated by sieve vibrator for 5 minutes until no test material or only an insignificant amount passed through the sieve. After this requirement was met for each sieve in the series, individual sieves were weighed with the retained sample and the first receiver pan and the final weights recorded. Duplicate test substance measurements were used to calculate the assay results. Precision (repeatability) was evaluated by duplicate measurements. The average percent of particles larger than 850 µm was 1.5%. The average percent of particles in the range of 300 µm - 850 µm was 11.2%. The average percent of particles in the range of 150 µm - 300 µm was 29.3%. The average percent of particles in the range of 75 µm - 150 µm was 29.3%. The average percent of particles in the range of 45 µm - 75 µm was 22.6%. The average percent of particles in the range of 32 µm - 45 µm was 5.5%. The average percent of particles smaller than 32 µm was 0.5%.

Description of key information

OECD Guideline 110

Additional information

The average percent of particles larger than 850 µm was 1.5%. The average percent of particles in the range of 300 µm - 850 µm was 11.2%. The average percent of particles in the range of 150 µm - 300 µm was 29.3%. The average percent of particles in the range of 75 µm - 150 µm was 29.3%. The average percent of particles in the range of 45 µm - 75 µm was 22.6%. The average percent of particles in the range of 32 µm - 45 µm was 5.5%. The average percent of particles smaller than 32 µm was 0.5%.