[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08.04.2021 - 15.06.2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2021

Report date:

2021

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Commission Regulation (EC) No 440/2008, C.2

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Reaction Products of 1,1,1-Trimethylol-propane-triacrylate with aziridine

Molecular formula:

C14H24O5N - C47H77O16N4

IUPAC Name:

Reaction Products of 1,1,1-Trimethylol-propane-triacrylate with aziridine

Test material form:

liquid: viscous

Specific details on test material used for the study:

Name of test substance: Emuldur 3643
Test substance No.: 19/0071-2
Batch identification: 27771924U0
Purity: 100 area-% UVCB
Homogeneity: Given, visual
Storage stability: Expiry Date: 15 Jul 2021

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

The following concentrations were tested: 0 (control), 4.6, 10, 22, 46 and 100 mg/L mg/L as nominal concentrations based on test substance mass without correction for content or purity.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Strain/clone: Daphnia magna STRAUS 1820
- Age at study initiation (range): 2 - 24 hours
- Source: supplied by Institut National de Recherche Chimique Appliquée, France, in 1978
- Feeding during test: None

Study design

Test type:

static

Water media type:

freshwater

Remarks:

Synthetic fresh water (Elendt M4) prepared according to OECD 202.

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 – 3.20 mmol/L

Test temperature:

19.2 - 20.2°C

pH:

7.5 – 8.5

Dissolved oxygen:

8.0 - 9.3 mg/L

Conductivity:

550 - 650 μS/cm

Nominal and measured concentrations:

0 (control), 4.6, 10, 22, 46 and 100 mg/L as nominal concentrations based on test substance mass without correction for content or purity. The analytical recovery efficiency was 100.4 – 101.1%. The effective concentrations in this report are based on the nominal concentrations.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass vessels
- Volume of solution: 50 mL
- Renewal rate of test solution: after 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The synthetic medium M4 is used for the culture and test. The medium is prepared on the basis of an ultrapure, deionized water.
- Acid capacity (up to pH 4.3): 0.80 - 1.00 mmoI/I
- Ca/mg ratio: about 4:1
- Conductivity: 550-650 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: about 157 – 694 lux at a wavelength of 400 – 750 nm; 16 hours light : 8 hours darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobility after 24 and 48 h

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

29.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In a 48 hours static acute toxicity study with Daphnia magna the EC50 of Emuldur based on the nominal concentrations was 29.6 mg/L.

Executive summary:

In a static acute toxicity laboratory study, water flea neonates (less than 24 h old) were exposed to Emuldur 3643 at concentrations of 4.6, 10, 22, 46 and 100 mg/L in M4 test medium. Daphnids were observed for immobility at 24 and 48 hours after start of exposure.