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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April - August 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Storage conditions: Controlled room temperature (15-25°C, ≤70% relative humidity), protected from light and humidity (stored in a tightly closed container)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: local wastewater treatment plant, ARA Birs (Birsfelden / Switzerland), which treats predominantly domestic sewage
- Concentration of sludge: 30 mg dry material per liter
- Preparation of Inoculum: The aerobic activated sewage sludge was washed three times by centrifugation, decantation of the supernatant (liquid phase) and resuspension of the solid material in tap water and finally in mineral medium. Aliquots of the homogenized final sludge suspension were weighed and dried (using a halogen moisture analyzer HS153 from Mettler Toledo) and the dry weight of the suspended solids was determined. Based on the sludge dry weight, a calculated amount of wet sludge was suspended in mineral medium to obtain a concentration equivalent to 4 g dry material per liter. During the holding period of two days prior to use, the sludge was aerated at room temperature. Prior to use, the dry weight of the sludge was again determined and the sludge was diluted with mineral medium. Defined volumes of this diluted activated sludge were added to the mineral medium in the test vessels to obtain a final concentration of 30 mg dry material per liter - Duration of test (contact time):
- 28 d
- Initial conc.:
- 82 mg/L
- Based on:
- ThOD
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Details on study design:
- A measured volume of inoculated mineral medium, containing a known concentration of test item (100 mg test item/L giving at least 50 - 100 mg Theoretical Oxygen Demand/L) as the nominal sole source of organic carbon was stirred in closed vessels at a constant temperature for 28 days. The biodegradation process consumed dissolved oxygen in the test vessel and generated carbon dioxide, which was absorbed by sodium hydroxide pellets. The consumption of oxygen was determined from the change in pressure in the incubation vessels. The amount of oxygen taken up by the microbial population during biodegradation of the test item (corrected for the uptake by blank inoculum, run in parallel) was expressed as a percentage of Theoretical Oxygen Demand (ThOD).
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 50 mg/l
- Preliminary study:
- Solubility Pre-Experiment:
Prior to test start, the solubility/dispersibility of the test item in mineral medium was checked, and the test item was found to be not soluble at a concentration of 102 mg/L. The pH of the dispersion with very fine, homogeneously distributed, blue particles was measured to be 7.5. Therefore, a test item stock solution could not be prepared and in the main test the test item was added directly to the test vessels. - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 30
- Sampling time:
- 28 d
- Remarks on result:
- other: not ready biodegradable
- Details on results:
- Test item:
The Biochemical Oxygen Demand (BOD) of the test item in the test media significantly increased from the test start until approximately day 7 of incubation, where biodegradation reached a mean value of 27 % (average of two replicates). Afterwards the biodegradation values remained in a plateau phase, with mean values in the range of 27 % to 31 %, until the end of the test, onday 28, when the mean biodegradation value of LZ 60102 amounted to 30 %.
At the end of the 10-day window, the mean biodegradation of the test item reached approximately 28 %. Therefore, the pass level for ready biodegradability, i.e. biodegradation of at least 60 % of the ThOD in a 10-day window within a 28-day period of the test, was not reached. In conclusion the test item LZ 60102 was considered as not readily biodegradable under the testconditions within 28 days. The obtained biodegradation value of 30 % may be regarded as
evidence of inherent, primary biodegradability.
Procedure and toxicity controls:
In the procedure controls, the average biodegradation of the reference item sodium benzoate was 68 % and 86 % reached by day 4 and day 14, respectively. Therefore, the suitability of the activated sludge was confirmed (≥ 60 % degradation was reached at day 14). At the end of the test (day 28), the average biodegradation was 89 %. In the toxicity control containing both LZ 60102 and the reference item sodium benzoate, the biodegradation amounted to 26 % by day 14 and reached 42 % at the end of the test period (day 28). Therefore, according to the test guidelines, the test item LZ 60102 was considered to have no inhibitory effect on the activity of activated sludge microorganisms at the tested concentration of 100 mg/L. - Remarks on result:
- not measured/tested
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item showed a biodegradation rate of 30% after 28 days and was therefore considered as not readily biodegradable under the test conditions.
- Executive summary:
The test item was investigated for its ready biodegradability in a manometric respirometry test according to the OECD Guideline for Testing of Chemicals, No. 301F and EU-Method C.4-D. The test was performed in closed vessels containing a known concentration of test item (100 mg test item/L giving 82 mg Theoretical Oxygen Demand/L) incubated at constant temperature (23 °C) for 28 days.
The percent of biodegradation of the test item was calculated based on the Theoretical Oxygen Demand (ThOD) of 0.82 mg O2/mg test item.
The Biochemical Oxygen Demand (BOD) of the test item in the test media significantly increased from the test start until approximately day 7 of incubation, where biodegradation reached a mean value of 27 % (average of two replicates). Afterwards the biodegradation values remained in a plateau phase, with mean values in the range of 27 % to 31 %, until the end of the test, on day 28, when the mean biodegradation value amounted to 30 %. At the end of the 10-day window, the mean biodegradation of the test item reached approximately 28 %. Therefore, the pass level for ready biodegradability, i.e. biodegradation of at least 60 % of the ThOD in a 10-day window within a 28-day period of the test, was not reached. In conclusion the test item was considered as not readily biodegradable under the test conditions within 28 days. The obtained biodegradation value of 30 % may be regarded as evidence of inherent, primary biodegradability.
Reference
Description of key information
The test item was investigated for its ready biodegradability in a manometric respirometry test according to the OECD Guideline for Testing of Chemicals, No. 301F and EU-Method C.4-D. The test was performed in closed vessels containing a known concentration of test item (100 mg test item/L giving 82 mg Theoretical Oxygen Demand/L) incubated at constant temperature (23 °C) for 28 days. The biodegradation values remained in a plateau phase, with mean values in the range of 27 % to 31 %, until the end of the test, on day 28, when the mean biodegradation value amounted to 30 %. At the end of the 10-day window, the mean biodegradation of the test item reached approximately 28 %. Therefore, the pass level for ready biodegradability, i.e. biodegradation of at least 60 % of the ThOD in a 10-day window within a 28-day period of the test, was not reached. In conclusion the test item was considered as not readily biodegradable under the test conditions within 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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