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EC number: 448-170-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experiment start and end date - 13 June 2003; Study completion date - 11 July 2003.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Specific details on test material used for the study:
- Identity: FAT 40810/A
Batch: WP 6/02
Purity: approx. 75 %
Appearance: Solid, dark brownish powder
Expiration date: 12 December 2010
Storage: At room temperature at about 20 °C - Radiolabelling:
- not specified
- Test temperature:
- Room temperature (approx. 20 °C)
- Details on study design: HPLC method:
- Principle
For the estimation of the adsorption coefficient, the HPLC-method was used. Therefore, an analytical column packed with a commercially available, cyanopropyl solid phase containing Iipophilic and polar moieties was used. A moderately polar stationary phase based on a silica matrix was used. While passing through the column along with the mobile phase, the test item interacts with the stationary phase. As a result of partitioning between mobile and stationary phases, the test item is retarded. The dual composition of the stationary phase having polar and non-polar sites allows for interaction of polar and non-polar groups of a molecule in a similar way as is the case for organic matter in soil or sewage sludge matrices. This enables the relationship between the retention time on the column and the adsorption coefficient on organic matter to be established.
Definitions
Kd: Distribution coefficient defined as the ratio of equilibrium concentrations Ce of a dissolved substance in a two-phase system consisting of a solid (soil or sewage sludge) and an aqueous phase; it is a dimensionless value varying with solid properties.
Kd = Csoil_sludge/Cwater .
C = Concentration of the test item at equilibrium (µg/g)
Koc: Distribution coefcient (Kd) normalised to the organic carbon content (OC) of a solid; particularly for non-ionised chemicals, it is an approximate indicator for the strength of adsorption between a substance and the solid and allows comparisons to be made between different chemicals.
KOC = Kd/OC (dimensionless)
The adsorption coefficient (KOC) is deduced from the capacity factor (k‘) using a calibration plot of log k’ versus log KOC of the selected reference items.
k’ = (tr – to)/to
tr = HPLC retention time of test or reference items (min.)
to = HPLC dead time (min.) (measurement of the retention time of sodium nitrate)
The adsorption coefficient (KOC) of the test item will be interpolated from the calibration curve which will be established by correlation of the log Koc values of various reference items with their log k‘ values. - Analytical monitoring:
- yes
- Details on sampling:
- not applicable (HPLC method)
- Details on matrix:
- see details on analytics (HPLC column)
- Details on test conditions:
- Test Item Solution
FAT 40810/A (15.2 mg) was dissolved in methanol (10 ml) to prepare a stock solution of 1520 µg/ml. The stock solution (1 ml) was diluted with methanol to 20 ml to obtain a standard solution of 76 µg/ml. The pH value of the test item solution was determined to be 7.3. The test item solution was injected three times.
Reference Solutions
The reference items were chosen from the list given in the OECD Guideline No. 121 and EC Directive C.19. Stock solutions of the reference items in methanol were prepared as described:
11.4 mg of the reference item phenol, 10.3 mg of the reference item Isoproturon, 11.1 mg of the reference item linuron, 10.4 mg of the reference item 2.4-DDT, 12.0 mg of the reference item pyrazophos and 11.5 mg of the reference item diclofop-methyl were each dissolved in 10 ml methanol. A combined reference solution was prepared by transferring 133 µl of the phenol stock solution, 190 µl of the Isoproturon, 120 µl of the linuron, 1430 µl of the pyrazophos, 275 µl of the diclofop-methyl and 1050 µl of the 2.4-DDT stock solution into one volumetric flask and diluting to a volume of 10 ml with a mixture of water and methanol (45:55; v/v). This reference solution was injected six times. Sodium nitrate (27.5 mg) was diluted to 100 ml with methanol to obtain a stock solution. The stock solution (100 µl) was diluted to 1 ml with a solvent mixture of water and methanol (45:55; v/v). This sodium nitrate solution was injected two times for the determination of the dead time of the HPLC system. - Type:
- log Koc
- Value:
- < 1.32
- Temp.:
- 20 °C
- Remarks on result:
- other: pH not measured
- Details on results (HPLC method):
- The determination of the adsorption coefficient (Koc) of FAT 40810/A was performed according to the OECD Guideline for the Testing of Chemicals, No. 121, Estimation of the Adsorption Coefficient (Koc) on Soil and Sewage Sludge using High Performance Liquid Chromatography (HPLC), January, 2001 and the EC Directive 2001/59, C.19. The test item solution was injected three times and the combined reference solution was injected six times. The log Koc was calculated using a regression curve (log k‘ vs. log Koc) and was found to be <1.32 which is equal to a Koc value of <21. The retention time of the test item solution was shorter than the retention time of the lowest retention time of the reference item phenol (Koc = 21; log Koc = 1.32). In the present study the reference items covered the range of log Koc 1.32 to log Koc 5.63.
The chromatography of the test item resulted in one main peak with a well-defined and reproducible retention time. The HPLC method was therefore considered to be suitable for the purpose of the study. Based on the chromatographic data, FAT 40810/A was considered to be stable during the test procedure. The retention times of the individual measurements of the reference items, test item and the dead time are given in provided tables. The capacity factor (k‘) was calculated for each reference item using the retention times. Log Koc was plotted versus log k for each component to obtain a regression curve. The tabulated values represent rounded mean results, which were obtained by calculation using the exact raw data. In conclusion, the log of the adsorption coefficient (Koc) of FAT 40810/A was estimated to be log Koc <1.32 which is equal to a Koc value of <21 using the HPLC method. This value indicates that FAT 40810/A is mobile and will not be absorbed by organic carbon in soil. - Validity criteria fulfilled:
- yes
- Conclusions:
- Result for FAT408190/A: log Koc <1.32 (equivalent to Koc <21)
- Executive summary:
The determination of the adsorption coefficient (Koc) of FAT 40810/A was performed according to the OECD Guideline No. 121 and the EC Directive 2001/59, C.19. in a GLP certified laboratory. HPLC was used for estimation of Koc and Log Koc. The log Koc was calculated using a regression curve (log k' vs. log Koc). Based on the findings of this study, the log Koc was determined to be <1.32 and Koc was determined to be <21.
Reference
Description of key information
The determination of the adsorption coefficient (Koc) of FAT 40810/A was performed according to the OECD Guideline No. 121 and the EC Directive 2001/59, C.19. in a GLP certified laboratory. HPLC was used for estimation of Koc and Log Koc. The log Koc was calculated using a regression curve (log k' vs. log Koc). Based on the findings of this study, the log Koc was determined to be <1.32 and Koc was determined to be <21.
Key value for chemical safety assessment
- Koc at 20 °C:
- 21
Additional information
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