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EC number: 421-300-1 | CAS number: 138564-59-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-04-08 to 1996-04-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 421-300-1
- EC Name:
- -
- Cas Number:
- 138564-59-7
- Molecular formula:
- C12H9N3O2S
- IUPAC Name:
- 5-methyl-2-[(2-nitrophenyl)amino]thiophene-3-carbonitrile
- Test material form:
- solid: particulate/powder
- Details on test material:
- 97.7%
Item code QA405K
Lilly lot no: 356MO1
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor and 356M01
- Purity test date: 1995-11-15
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature in a tightly sealed condition
- Solubility and stability of the test substance in the solvent/vehicle:
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: 0.9g of test substance was dissolved in acetone to prepare a 6 mg test substance/ml stock solution
- Final dilution of a dissolved solid, stock liquid or gel: The final set of concentrations were prepared by diluting the stock solution with acetone/chilled conditioned well water to a final volume of approximately 134 L
FORM AS APPLIED IN THE TEST (if different from that of starting material)
Nominal test concentrations were 0 (water and acetone control), 70, 800, 1200, 1500, and 1800 µg/L
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 (water and acetone control), 70, 800, 1200, 1500, and 1800 µg/L
- Sampling method: Samples for analysis were collected at test initiation and termination. In addition, samples of aged and newly prepared test solutions were collected at each renewal period. Each collected sample was 7.5 ml and was diluted with 2.5 ml of methanol. Samples were collected prior to the daily renewal
- Sample storage conditions before analysis: Samples were immediately submitted for analysis.
Test solutions
- Vehicle:
- yes
- Remarks:
- Acetone
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: Water and acetone control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone/conditioned distileed well water
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 298.5 µI acetone/L of dilution water
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Juvenile rainbow trout
- Strain: Onchorhynchus mykiss
- Source: Hatched at the Eli Lilly Environmental Toxicology Laboratory on December 8, 1995, from eyed eggs supplied by Trout Lodge in McMillin, WA
- Age at study initiation (mean and range, SD): 134 days
- Length at study initiation (length definition, mean, range and SD): Averaged 64 ± 4.8 mm and ranged between 55 and 69 mm, total length
- Weight at study initiation (mean and range) : Averaged 2.20 g and ranged from 1.29 to 2.79 g.
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): Same as test except for the addition of aeration
- Type of food during acclimation: Dry pelleted formulation daily (Trout feed, Formula No.1, manufactured by Zeigler Brothers, Gardners, PA).
- Feeding frequency during acclimation: Daily until 48 hours before the test
- Health during acclimation (any mortality observed): <0.1%
FEEDING DURING TEST
- The fish were not fed during the test
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 4 d
- Post exposure observation period:
- Fish were immediately euthanized following the conclusion of the test.
Test conditions
- Hardness:
- 137mg/l as CaCO3
- Test temperature:
- 10.4-11.6 degrees Celsius over the exposure period
- pH:
- 7.3-7.7 over the exposure period of the test
- Dissolved oxygen:
- 10.3-11.9 mg/L over the exposure period
- Conductivity:
- 256 umhos
- Nominal and measured concentrations:
- Nominal: 0 (water and acetone control), 70, 800, 1200, 1500, and 1800 µg/L
Measured: Averages were 0, 77, 885, 1364, 1703, and 2076 µg/L respectively - Details on test conditions:
- TEST SYSTEM
- Test vessel: Large aquarium
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Glass, >134 L, 134L
- Aeration: None
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: Unspecified
- No. of vessels per concentration (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.02 g/L approximately
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Well water
- Alkalinity: 122 mg/L as CaCO3
OTHER TEST CONDITIONS
- Adjustment of pH: The dilution water was conditioned
- Photoperiod: 16/8 light/dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Fish behavior on a scale was measured at test intiation and after approximately 2, 24, 48, 72, and 96 hours of exposure.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Determined by standard procedure
- Range finding study: None - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: Median lethal concentration
- Effect conc.:
- 1 279 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 77 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Details on results:
- - Behavioural abnormalities: At concenttations of >=885 µg/L, the trout exhibited exposure-related signs of toxicity including hypoactivity, impaired swimming, prostration, and death.
- Mortality of control: None - Results with reference substance (positive control):
- No reference substance was used.
- Reported statistics and error estimates:
- The LC50 value was calculated using the probit procedure (SAS 1990). All values expressed as "x ± y" represent means± standard deviations and were calculated using either SAS (1990) or a hand calculator.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- This study validly assessed the short term aquatic toxicity of the test substance according to the specified guideline. The 96-hour median lethal concentration of the test substance to rainbow trout was 1279 µg/L with 95% confidence limits of 1073 to 1458 µg/L. The slope of the concentration-mortality curve at 96 hours was 9.5. The no-observed effect concentration to rainbow trout was an average assayed concentration of 77 µg/L.
- Executive summary:
The study was carried out in accordance with EU method C.1. The 96 hour median lethal concentration was 1279ug/l and the NOEC was 77ug/l.
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