Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 221-391-6 | CAS number: 3084-40-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP and appropriate guidelines
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2400 (Acute Eye Irritation)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Japanese Ministry of Agriculture, Forestry and Fisheries, Test Data for Registration of Agricultural Chemicals, Eye Irritation (2-1-5), 12 Nohsan No. 8147, Agricultural Production Bureau, November 24, 2000.
- Deviations:
- no
- Principles of method if other than guideline:
- n/a
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Diethyl (hydroxymethyl)phosphonate
- EC Number:
- 221-391-6
- EC Name:
- Diethyl (hydroxymethyl)phosphonate
- Cas Number:
- 3084-40-0
- Molecular formula:
- C5H13O4P
- IUPAC Name:
- diethyl (hydroxymethyl)phosphonate
- Reference substance name:
- Diethoxyphosphoryl methanol
- IUPAC Name:
- Diethoxyphosphoryl methanol
- Test material form:
- other: liquid
- Details on test material:
- E06-16 (Lot No. 1041-37-6, T# 184), a colotless liquid. Stored in dark at room temp. (Approximately 25oC).
The test material was stable for the duration of the study.
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Animals for this study were selected from a stock supply of healthy adult rabbits.
Number of animals - 3 male rabbits
Age of start of treatment- 35 to 42 weeks
Bodyweight range of 3.01 to 3.50 kg
Identification- A numbered tag placed through the edge of one ear.
Husbandary: Daily average animal room temperature and relative humidity within the range 16 to 20°C, and 40 to 70%. respectively.
Light: 12 hr artificial light 12 hr dark.
Accommodation: Individually in labelled cages. Each cage was identified by a coloured label displaying the study number and animal number.
All rabbits were acclimatised to the experimental environment for a period of approximately 15 weeks prior to the start of the study.
Diet and water: Each animal was housed individually in a plastic cage with perforated floors and was offered
150 g of a standard laboratory rabbit diet per day; drinking water was provided ad libitum.
The batch of diet used for the study is analysed for nutrients, possible contaminants and
micro-organisms likely to be present in the diet and which, if in excess of specified amounts,
might have an undesirable effect on the test system. A dietary supplement of hay was offered
during acclimatisation until two days prior to dose instillation; for the remainder of
acclimatisation and throughout the study observation period wholemeal bread was offered.
Twice weekly a supplement of fresh chopped vegetables was given.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 0.1 mL of the test substance
- Duration of treatment / exposure:
- 24h
- Observation period (in vivo):
- 0, 1, 24, 48 and 72 hours after test material administration.
All rabbits were observed at least once each day for mortality and moribundity. - Number of animals or in vitro replicates:
- 3 treated animals.
- Details on study design:
- The eyes of each animal were examined prior to instillation of the test substance to ensure that there was no pre-existing corneal damage, iridial inflammation or conjunctival irritation. Each animal was gently restrained. The dose (0.1 mL) was instilled into the right eye by pulling the lower eyelid away from the eye ball to form a cup into which the test substance was dropped. The left eye remained untreated and served as a control. The eyelids were then gently held together for one second before releasing. The treated and control eyes of each test rabbit were examing using a penlight. The cornea was observed for presence and/or degree of opacity; the iris for deepened rugae, congestion, swelling and circumcorneal injection, and reaction to light; and the conjunctiva for redness and chemosis.
Evaluation of the results: For each animal, mean scores for corneal opacity, iris lesions, conjunctival redness and chemosis were calculated by addingthe scores at 24, 48 and 72 hours and dividing by three. The irritation potential of the test material was assessed according to the system of Kay and Calandra, 1962.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 1h
- Score:
- 1.7
- Max. score:
- 1.7
- Reversibility:
- fully reversible within: 24h
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 1h
- Score:
- 6
- Max. score:
- 6
- Reversibility:
- fully reversible within: 72h
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24 h
- Score:
- 1.3
- Max. score:
- 1.3
- Reversibility:
- fully reversible within: 72h
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 48 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 48 h
- Score:
- 0.7
- Max. score:
- 0.7
- Reversibility:
- fully reversible within: 72h
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Irritant / corrosive response data:
- Injection of the conjunctival blood vessels or a crimson-red conjunctival appearance was
apparent in all animals one hour after instillation persisting in one animal for twenty-four
hours and in another for forty-eight hours. Very-slight or slight discharge was evident in all
animals one hour after instillation and iritis was apparent in one case at this time. The treated
eyes of all animals were overtly normal 72 hours after instillation.
Instillation of the test material gave rise to no initial pain response. - Other effects:
- There was no sign of toxicity or ill health in any rabbit during the observation period.
Any other information on results incl. tables
n/a
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- The test material is judged to be minimally irritating.
- Executive summary:
Three rabbits were each administered a single ocular dose of a volume of 0.1 mL of the test substance and observed for four days after instillation.
Injection of the conjunctival blood vessels or a crimson-red conjunctival appearance was apparent in all animals one hour after instillation persisting in one animal for twenty-four hours and in another for forty-eight hours. Very-slight or slight discharge was evident in all animals one hour after instillation and iritis was apparent in one case at this time. The treated eyes of all animals were overtly normal 72 hours after instillation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.