N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

literature data

Data source

Reference

Reference Type:

publication

Title:

Marine biodegradability and ecotoxicity of solvents for CO2-capture of natural gas

Author:

Ingvild Eide-Haugmo, Odd Gunnar Brakstad, Karl Anders Hoff, Eirik Falck da Silva ,
Hallvard F. Svendsen,

Year:

2012

Bibliographic source:

www.elsevier.com/locate/ijggc

Report date:

2012

Materials and methods

Test guideline

Guideline:

other: ISO/DIS guideline 10253

GLP compliance:

no

Test material

Test material information

Test material form:

not specified

Details on test material:

CAS 6711-48-4, 95% purity

Specific details on test material used for the study:

The test chemical was obtained commercially (Sigma–Aldrich or Fluka), with a purity >95%.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The choice of test concentrations was based on a preliminary test with ten-fold
dilutions of the test substance (10–10,000 mg/l). As controls, algae
were also inoculated to algal culture medium without test substance.

Test organisms

Test organisms (species):

Skeletonema costatum

Study design

Test type:

not specified

Water media type:

saltwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

20 ± 2 ◦C

Nominal and measured concentrations:

10–10,000 mg/l

Details on test conditions:

TEST SYSTEM

- No. of organisms per vessel: 3×10000 cells/ml
- No. of vessels per concentration (replicates): 3

OTHER TEST CONDITIONS
- Photoperiod: constant light
- Light intensity and quality: 60 - 120 (µE/s/m2)
- Salinity (for marine algae):


The ecotoxicity was determined by a marine phytoplankton test,
conducted according to ISO/DIS guideline 10253, using the algal
species Skeletonema costatum (ISO, 1999).
Concentration series of each chemical was prepared in triplicate
in a seawater-based algal culture medium, and the algal in
exponential growth phase was inoculated in each test solution at
algal concentrations of approximately 3 × 103 cells/ml. The choice
of test concentrations was based on a preliminary test with ten-fold
dilutions of the test substance (10–10,000 mg/l). As controls, algae
were also inoculated to algal culture medium without test substance.
All test tubes were incubated with agitation under constant
light intensity (60–120 E/s/m2) and a temperature of 20
±2 ◦C for 72 h.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: (95% confidenbce interval 18-22 mg/L)

Reported statistics and error estimates:

From these parameters the effective concentrations
(mg/l) of test item inhibiting algal growth by 50% (EC50) relative
to the control cultures, were calculated with a 95% confidence
interval using the ecotoxicity calculation program TOXEDO (Water
Quality Institute, Hørsholm, Denmark).

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

In a publication from Eide-Haugmo et al. 2012, a algae grwoth inhibition test with Skeletonema costatum according to ISO/DIS 10253 was performed. The EC50 for the test item was 20 mg/L.