Lithium dihydrogen 5-sulphonatoisophthalate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Principles of method if other than guideline:

The study was performed as described in:
Ewell, WS, Gorsuch, JW, Kringle, RO, Robillard, KA, Spiegel, RC (1986). Simultaneous evaluation of the acute effects of chemicals on seven aquatic species. Environ. Toxicol. Chem. 5:831-840.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Sodium hydrogen-5-sulphoisophthalate

EC Number:

228-845-2

EC Name:

Sodium hydrogen-5-sulphoisophthalate

Cas Number:

6362-79-4

Molecular formula:

C8H6O7S.Na

IUPAC Name:

Sodium 3,5-dicarboxybenzenesulfonate

Constituent 2

Reference substance name:

288-845-2

IUPAC Name:

288-845-2

Constituent 3

Reference substance name:

Sodium-3,5-dicarboxybenzenesulfonate

IUPAC Name:

Sodium-3,5-dicarboxybenzenesulfonate

Constituent 4

Reference substance name:

5-(Sodiosulfo)isophthalic acid

IUPAC Name:

5-(Sodiosulfo)isophthalic acid

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material: 5-(Sodiosulfo)isophthalic Acid
- Appearance/Physical form: powder
- Formula Weight: 268 g/mol
- Molecular Formula: C8H6O7S-1Na
- Purity: >92% (assay)
- Impurities: sodium sulfate,- Lot/batch No.: ARK BA103-81; X-17007-226

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Chemicals known to be soluble at 100mg/l were added directly to dilution water.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

All test organisms were acclimated to the control diluent water in breeding/rearing tanks. Food was withheld for the 24 h preceding the start of testing. Ten juveniles, as uniform in size as possible, were collected from the breeding colonies. The first and second larval instar were used.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Test conditions

Test temperature:

20 ± 1 °C

pH:

6.5 to 8.5

Dissolved oxygen:

Maintained at 40% or greater of the starting level. If levels fell below this, the test was repeated with 0.05 l/min of aeration.

Nominal and measured concentrations:

Nominal concentrations were employed. The material is completely water soluble. In addition, based on analytical measurements, stable 100 mg/l concentrations of the test material were maintained in an algal toxicity study. Thus, the use of nominal concentrations is justified.

Details on test conditions:

TEST SYSTEM
- Test vessel: Seamless glass 30.5 cm cuboidal Pyrex jars of 20 l capacity
- Type: open
- Aeration: if required
- No. of organisms per vessel: 10 of each species
- No. of vessels per concentration: 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.5 g wet weight/l (fish)

TEST MEDIUM / WATER PARAMETERS (Typical values)
- Source/preparation of dilution water: Industrial service water from Lake Ontario, Rochester, NY (USA). Filtered through activated carbon and chlorinated.
- Total organic carbon: 1.8 mg/l
- Metals: Measured routinely and all within acceptable levels.
- Chlorine: 6.6 µg/l (residual)
- Total dissolved solids: 180 mg/l
- Total hardness (CaCO3): 130 mg/l
- pH: 7.4

Photoperiod: 16 h light
The air-water interface of each tank received approximately 50 ft-c of cool-white fluorescent light.

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

The test material did not affect daphnia magna.

Results with reference substance (positive control):

See the publication of Ewell et al. (1986) for reference substance information.

Reported statistics and error estimates:

LC50 values were estimated using a computer program of Stephan (Methods for calculating an LC50: Aquatic toxicology and hazard evaluation. In F.L. Mayer and J.L. Hameling, eds., STP 634. American Society for Testing and Materials, Philadelphis, PA, pp 65-84).

Applicant's summary and conclusion

Conclusions:

The acute aquatic LC50 of the test material to daphnia magna was > 100 mg/l.

Executive summary:

Exposure of 10 daphnia magna for 96 h to a 100 mg/l solution of the test material produced no mortality.