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EC number: 283-403-6 | CAS number: 84625-29-6 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Capsicum annuum, Solanaceae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Examination of Chili Pepper and Nutmeg Oleoresins Using the Salmonela / Mammalian Microsome Mutagenicity Assay
- Author:
- Buchanan R L
- Year:
- 1 981
- Bibliographic source:
- Journal of Food Science, 47, 330-333.
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Capsicum annuum, ext.
- EC Number:
- 283-403-6
- EC Name:
- Capsicum annuum, ext.
- Cas Number:
- 84625-29-6
- Molecular formula:
- Not available since an UVBC substance.
- IUPAC Name:
- Capsicum annuum, ext.
- Test material form:
- liquid
Constituent 1
Method
- Target gene:
- Histidine-requiring gene in Salmonella typhimurium
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium TA 1535
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Type and composition of metabolic activation system:
- source of S9 : livers of rats induced with phenobarbital.
- concentration or volume of S9 mix in the final culture medium: 0.5 mL/plate - Test concentrations with justification for top dose:
- 2.5, 25, 250, 2500 and 25000 µg/plate.
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO.
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N-nitro-N-nitrosoguanidine and picrolinic acid
- Remarks:
- Without metabolic activation.
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- Remarks:
- With metabolic activation.
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration: 4
- Number of independent experiments: 1
METHOD OF TREATMENT/ EXPOSURE:
- Cell density at seeding (if applicable): Not specified.
- Test substance added in agar (plate incorporation) at a level of 0.5 mL/plate.
TREATMENT AND HARVEST SCHEDULE:
- Exposure duration/duration of treatment: 48 hr at 37ºC.
METHODS FOR MEASUREMENT OF CYTOTOXICITY
- Method: background growth inhibition.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- The test substance chili pepper oleoresin was found as no mutagenic in a Salmonella / mammalian microsome mutagenicity assay.
- Executive summary:
The test substance chili pepper oleoresin was tested for in vitro mutagenicity using the Salmonella / mammalian microsome mutagenicity assay similar to OECD Guideline 471. The experiments were performed with at least four replications, using Salmonella typhimurium strains TA 98, TA 100, TA 1535 and TA 1538 in the presence and absence of metabolic activation (S9 fraction prepared from the livers of rats). The test item was formulated in 0.1 mL DMSO as vehicle and concentrations of 2.5, 25, 250, 2500 and 25000 µg/plate were used in the assay. Vehicle control and appropriate positive controls (N-methyl-N-nitro-N-nitrosoguanidine and picrolinic acid without metabolic activation and 2-acetylamino-flourene with metabolic activation) were tested simultaneously. The test substance did not produce an increased reversion rate, while negative and positive controls were within expected ranges. These data indicates that the test substance is not mutagenic.
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