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EC number: 215-566-6 | CAS number: 1332-07-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The micronucleus test was performed according to Schmid [51]. The animals were treated at 0 and 24 h, and bone-marrow smears were prepared at 30 h. Usually, 4 mice (2 male, 2 female) were used for each of 3 doses and 1 control. Slides were coded, and 1000 polychromatic erythrocytes were scored per mouse. Significance was calculated according to the Kastenbaum-Bowman tables
51] Schmid, W., The micronucleus test for cytogenetic analysis, in: A. Hollaender (Ed.), Chemical Mutagens, Vol. 4, Plenum, New York, 1976, pp. 31-53. - GLP compliance:
- not specified
- Type of assay:
- other: mammalian somatic cell study
Test material
- Reference substance name:
- Zinc sulphate
- EC Number:
- 231-793-3
- EC Name:
- Zinc sulphate
- Cas Number:
- 7733-02-0
- Molecular formula:
- H2O4S.Zn
- IUPAC Name:
- zinc sulfate
- Details on test material:
- Source: Merck Co., Darmstadt (Germany);
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Details on species / strain selection:
- Source: S. Ivanovas GmbH and Co., Kisslegg/Allgäu (Germany)
diet: ad libitum, standard chow (Altromin GmbH, Lage, Germany)
water. ad libitum - Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: unspecified
- Duration of treatment / exposure:
- Bone-marrow smears were prepared at 30 h.
- Frequency of treatment:
- The animals were treated at 0 and 24 h.
- No. of animals per sex per dose:
- Usually, 4 mice (2 male, 2 female) were used for each of 3 doses and 1 control.
- Control animals:
- yes, plain diet
Examinations
- Tissues and cell types examined:
- Polychromatic erythocytes
- Details of tissue and slide preparation:
- Bone-marrow smears were prepared at 30 h.
- Evaluation criteria:
- not specified
- Statistics:
- Significance was calculated according to the Kastenbaum-Bowman tables.
Results and discussion
Test results
- Key result
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- not specified
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- not examined
Any other information on results incl. tables
Table 1: Results of the mirconucleus test on mouse bone marrow
Compound | surviving/treated mice | Dose | |||
mg/kg | mmole/kg | Route of application | Micronucleated PEa(‰) | ||
Zinc sulfatec | 3/4 | 2x86.3 | 2x0.3 | i.p. | 2.9 |
4/4 | 2x57.5 | 2x0.2 | i.p. | 1.9 | |
4/4 | 2x28.8 | 2x0.1 | i.p. | 1.7 | |
4/4 | 0 | i.p. | 1.9 | ||
aPolychromatic erythocytes cHanks' balances salt solution (HBSS) |
Applicant's summary and conclusion
- Conclusions:
- Zinc sulfate showed no genetic activity in the micronucleus test.
- Executive summary:
The in vivo micronucleus test on mouse bone marrow was performed according to Schmid and international standard methods. The animals were treated at 0 and 24 h with zinc sulfate, and bone-marrow smears were prepared at 30 h. Usually, 4 mice (2 male, 2 female) were used for each of 3 doses and 1 control. Slides were coded, and 1000 polychromatic erythrocytes were scored per mouse. Significance was calculated according to the Kastenbaum-Bowman tables.
Zinc sulfate showed no genetic activity in the micronucleus test.
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