Registration Dossier
Registration Dossier
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EC number: 201-126-0 | CAS number: 78-59-1
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The substance with high probability acutely not harmful to aquatic invertebrates.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 224 mg/L
Additional information
Experimental data are available for the assessment of the short-term toxicity of isophorone to aquatic invertebrates. Under supervision of the Japanese Ministry of the Environment an acute daphnia test according to OECD 202 was conducted under GLP and published in 1996. The test species was Daphnia magna. The 48-h EC50 was determined to be 224 mg/L (nominal, NITE, 1996).
In addition, the substance was tested in a static short-term toxicity test with Daphnia magna as a test organism in the publication of Le Blanc (1980). The study was conducted according to the methods of US EPA, EPA-660/3-75-009 (1975). The 48-h EC50 was determined to be 120 mg/L (nominal, Le Blanc, 1980). The EC50 value in the publication of Le Blanc (1980) appears to be more sensitive than the EC50 value in the OECD 202 study from NITE (1996). However, in the publication of Le Blanc the procedures are reported but not assigned to substances itself, therefore, it is not clear if the vehicle was used in this study. Thus, it is not clear if the toxicity of the substance can be due to vehicle. Therefore, the 48-h EC50 value from the GLP OECD 202 study are considered as a key value for the assessment of isophorone (CAS 78-59-1).
In both studies the test concentrations were not analytically verified. However, the stability of the substance was analytically verified in various ecotoxicity studies with fish (Cairns and Nebeker, NITE, see IUCLID Ch. 6.1.1) and in a study with algae (NITE, see IUCLID Ch. 6.1.5). Moreover, the substance appeared to be stable during endocrine studies (see IUCLID Ch. 6.1.8).
Overall, it can be concluded that the substance with high probability acutely not harmful to aquatic invertebrates.
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